Team:KULeuven/26 August 2008

From 2008.igem.org

(Difference between revisions)
(→Wet Lab)
(→Wet Lab)
Line 5: Line 5:
=== Wet Lab ===
=== Wet Lab ===
-
Looks like we've finally nailed the transduction-PCR. The photo of our gel looks very promissing, tomorrow we'll discuss it with our advisors, but we think our cells should be fine!
+
* Looks like we've finally nailed the transduction-PCR. The photo of our gel looks very promissing, tomorrow we'll discuss it with our advisors, but we think our cells should be fine!
 +
* We cut B0015 and F1610+R0011 with ''Xba''I and ''Eco''RI. There is something wrong with F1610+R0011, so we have to do this again.
 +
* The following parts were elektroporated: K145001+pSB1A2 and K145151+pSB1A2.
 +
* We made liquid cultures of some BioBricks.
 +
* We did a PCR to test the ligations. We are not sure of the ligations J23116+B0032 and ''J23022+R0040''. The other ones seem to be OK.
=== Dry Lab ===
=== Dry Lab ===

Revision as of 19:10, 26 August 2008

  dock/undock dropdown  

Contents

Lab Work

Wet Lab

  • Looks like we've finally nailed the transduction-PCR. The photo of our gel looks very promissing, tomorrow we'll discuss it with our advisors, but we think our cells should be fine!
  • We cut B0015 and F1610+R0011 with XbaI and EcoRI. There is something wrong with F1610+R0011, so we have to do this again.
  • The following parts were elektroporated: K145001+pSB1A2 and K145151+pSB1A2.
  • We made liquid cultures of some BioBricks.
  • We did a PCR to test the ligations. We are not sure of the ligations J23116+B0032 and J23022+R0040. The other ones seem to be OK.

Dry Lab

Alright, here we go. The two parallel schemes for building the system:

new key-lock
old key-lock, parallel scheme

General meeting with the team decided upon some important/emergency building-stuff :)

We've started on thinking about the ethical part of our project. We would start from the film "I, Robot" and then focus further on the laws of robotics. Benjamien and Hanne did a lot of research on probiotics, ethics and genetic engineering.

Modeling

Some more stochastic stuff

Wiki

General modeling page updates.

Remarks

<< return to notebook return to homepage >>
< previous friday ← yesterday tomorrow → next monday >

Retrieved from "http://2008.igem.org/Team:KULeuven/26_August_2008"