Team:KULeuven/28 August 2008

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(Difference between revisions)
(New page: {{:Team:KULeuven/Tools/Header}} == Lab Work == === Wet Lab === * The ligations with B0015 (C0012+B0015, C0060+B0015, C0040+B0015, K145001+B0015, K145151+B0015, K45015+B0015) were electr...)
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* The ligations of K145001 and K145151 with pSB1A2 were MiniPrepped and then we did a PCR to test the ligation.
* The ligations of K145001 and K145151 with pSB1A2 were MiniPrepped and then we did a PCR to test the ligation.
* The digests of R0011, R0040+J23022, C0062 and B0033 were put on gel and purified.
* The digests of R0011, R0040+J23022, C0062 and B0033 were put on gel and purified.
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* Some more digests were made, but this time we cut with ''Spe''I, ''Xba''I and ''Pst''I. We cut B0015 as insert and C0060, K145015, C0012, C0040 were cut as vector.  
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* Some more digests were made, but this time we cut with ''Spe''I, ''Xba''I and ''Pst''I. We cut B0015 as insert and C0060, K145015, C0012 and C0040 were cut as vector.
=== Dry Lab ===
=== Dry Lab ===

Revision as of 19:17, 28 August 2008

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Contents

Lab Work

Wet Lab

  • The ligations with B0015 (C0012+B0015, C0060+B0015, C0040+B0015, K145001+B0015, K145151+B0015, K45015+B0015) were electroporated again, but now they were plated on an ampicillin plate.
  • A glycerol stock was made of the succeeded ligations.
  • The new parts arrived today. They were also plated.
  • The ligations of K145001 and K145151 with pSB1A2 were MiniPrepped and then we did a PCR to test the ligation.
  • The digests of R0011, R0040+J23022, C0062 and B0033 were put on gel and purified.
  • Some more digests were made, but this time we cut with SpeI, XbaI and PstI. We cut B0015 as insert and C0060, K145015, C0012 and C0040 were cut as vector.

Dry Lab

Modeling

Wiki

Remarks

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