Team:Hawaii/Notebook/2008-08-31

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(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS=== :<strong> Grace</strong> [[Image:083108REdigests.j...)
(→Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS)
Line 7: Line 7:
[[Image:083108REdigests.jpg|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 1.5 hours.]]
[[Image:083108REdigests.jpg|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 1.5 hours.]]
:* Ran RE digests from last night on gel
:* Ran RE digests from last night on gel
 +
::*plac+rbs band not visible; will ligate using plac and rbs parts (instead of plac+rbs part)
:* Extracted bands from gel
:* Extracted bands from gel
:* Ligated:
:* Ligated:
-
::* plac+rbs and C0012 vector
+
::* plac and rbs (B0034) and C0012 vector
::* J33207 and BB-pRL1383a
::* J33207 and BB-pRL1383a
:* Transformed into DH5&alpha; cells
:* Transformed into DH5&alpha; cells
-
::* Plated BB-pRL1383a+J33207 on LB+sp<sub>100</sub>+X-gal and LB+sp<sub>100</sub>+sm<sub>100</sub>+X-gal+IPTG to verify if IPTG is necessary for blue/white screening in this strain
+
::* Plated BB-pRL1383a+J33207 on selective media with and without IPTG to verify if IPTG is necessary for blue/white screening in this strain
 +
 
= Discussion =
= Discussion =

Revision as of 01:29, 1 September 2008

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Things we did today

Wetlab work

Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS

Grace
EtBr stained 0.8% agarose gel ran at 60V for 1.5 hours.
  • Ran RE digests from last night on gel
  • plac+rbs band not visible; will ligate using plac and rbs parts (instead of plac+rbs part)
  • Extracted bands from gel
  • Ligated:
  • plac and rbs (B0034) and C0012 vector
  • J33207 and BB-pRL1383a
  • Transformed into DH5α cells
  • Plated BB-pRL1383a+J33207 on selective media with and without IPTG to verify if IPTG is necessary for blue/white screening in this strain

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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