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Team:KULeuven/Freezer
From 2008.igem.org
(Difference between revisions)
(→Parts stored in the fridge in the lab (4°C)) |
(→Parts stored in the fridge in the lab (4°C)) |
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|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_J23116 J23116]||BBa_J61002 (Ap)||Constitutive promoter ||+/- 48||328.8||52||August 08 | |[http://partsregistry.org/Part:BBa_J23116 J23116]||BBa_J61002 (Ap)||Constitutive promoter ||+/- 48||328.8||52||August 08 | ||
| - | |||
| - | |||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0011 R0011]||pSB1A2||LacI regulated promoter ||+/- 40||69.8||42||July 29 | |[http://partsregistry.org/Part:BBa_R0011 R0011]||pSB1A2||LacI regulated promoter ||+/- 40||69.8||42||July 29 | ||
| Line 120: | Line 118: | ||
|[http://partsregistry.org/Part:BBa_R0084 R0084]||pSB1A2||OmpF promoter||+/- 40||32.0||14||July 23 | |[http://partsregistry.org/Part:BBa_R0084 R0084]||pSB1A2||OmpF promoter||+/- 40||32.0||14||July 23 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_R1052 R1052]||pSB1K0||434cI regulated promoter||+/- 15||27.5||60||September 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0060 C0060]||pSB1A2||Lactonase||30||155.3||54||July 31 | |[http://partsregistry.org/Part:BBa_C0060 C0060]||pSB1A2||Lactonase||30||155.3||54||July 31 | ||
| Line 135: | Line 133: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_E0240 E0240]||pSB1A2||GFP generator||+/- 44||281||59||August 30 | |[http://partsregistry.org/Part:BBa_E0240 E0240]||pSB1A2||GFP generator||+/- 44||281||59||August 30 | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0056 C0056]||pSB1A2||cI represor||+/- 45||419.8||64||August 30 | |[http://partsregistry.org/Part:BBa_C0056 C0056]||pSB1A2||cI represor||+/- 45||419.8||64||August 30 | ||
| Line 149: | Line 141: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_E0040 E0040]||pSB1A2||GFP||+/- 48||305.8||67||August 30 | |[http://partsregistry.org/Part:BBa_E0040 E0040]||pSB1A2||GFP||+/- 48||305.8||67||August 30 | ||
| - | |||
| - | |||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0053 R0053]||pSB1A2||p22 cII regulated promotor||+/- 25||72.1||69||August 30 | |[http://partsregistry.org/Part:BBa_R0053 R0053]||pSB1A2||p22 cII regulated promotor||+/- 25||72.1||69||August 30 | ||
| Line 202: | Line 192: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||GFP with LVA tag||+/- 46||152.7||2A||August 08 | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||GFP with LVA tag||+/- 46||152.7||2A||August 08 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||GFP+LVA||+/- 48||257||4E||August 30 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB||+/- 48||204.6||3E||August 30 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||T7 without UmuD||+/- 48||414.8||5E||August 30 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB||+/- 48||188.4||6E||August 30 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||57.8||5B||August 28 | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||57.8||5B||August 28 | ||
| Line 329: | Line 327: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||14.1||41||August 12 | |[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||14.1||41||August 12 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_K145013 K145013] (aLuxI)|| ''Eco''R I||''Spe'' I||Insert||660||+/-25||20.8||53||September 05 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_K145150 K145150] (promoter)|| ''Eco''R I||''Spe'' I||Insert||110||+/-25||9.2||54||September 05 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||24.3||48||August 12 | |[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||24.3||48||August 12 | ||
| Line 370: | Line 372: | ||
|[http://partsregistry.org/Part:BBa_R0040 R0040]|| ''Eco''R I||''Spe'' I||Insert||70||10||0.8||52||September 2 | |[http://partsregistry.org/Part:BBa_R0040 R0040]|| ''Eco''R I||''Spe'' I||Insert||70||10||0.8||52||September 2 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |||
|[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||61||25||3.4||28||July 29 | |[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||61||25||3.4||28||July 29 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| Line 400: | Line 401: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-28||2.6||75||August 25 | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-28||2.6||75||August 25 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-25||3.8||62||September 05 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||311||+/-28||3.6||74||August 27 | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||311||+/-28||3.6||74||August 27 | ||
Revision as of 13:39, 5 September 2008
dock/undock dropdown
Parts stored in the fridge in the lab (4°C)
| MiniPrep Products: Parts |
|---|
| Part | Plasmid | Description | µl left | ng/µl | Box | Date |
|---|---|---|---|---|---|---|
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 10 | 112.3 | 7 | July 24 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 20 | 130.3 | 8 | July 24 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 303.1 | 9 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 20 | 213.6 | 10 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 48 | 311.0 | 11 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 30 | 214.0 | 12 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 209.5 | 13 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 63.0 | 6 | July 23 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | RBS(medium) | +/- 5 | 51.0 | 57 | August 11 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | RBS(medium) | +/- 20 | 47.2 | 55 | August 27 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | RBS(medium) | +/- 40 | 27.0 | 56 | August 27 |
| [http://partsregistry.org/Part:BBa_B0033 B0033] | pSB1AK3 | RBS | +/- 50 | 36.5 | 43 | August 27 |
| [http://partsregistry.org/Part:BBa_B0033 B0033] | pSB1AK3 | RBS | +/- 23 | 38.4 | 44 | August 27 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 20 | 73.8 | 47 | July 24 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 30 | 95.1 | 48 | July 24 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 40 | 104.0 | 49 | July 29 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1AK3 | RBS | +/- 15 | 57 | 50 | August 27 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1AK3 | RBS | +/- 50 | 29.6 | 51 | August 27 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI | +/- 30 | 133.1 | 20 | July 29 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI repressor | +/- 40 | 146.4 | 22 | July 29 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI repressor | +/- 40 | 203.1 | 21 | July 29 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | pSB1A2 | TetR repressor | +/- 20 | 177.1 | 4 | July 24 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | pSB1A2 | TetR repressor | +/- 30 | 161.7 | 5 | July 24 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | pSB1A2 | lactonase production | +/- 48 | 84.2 | 53 | August 11 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | pSB1A2 | LuxR repressor | +/- 48 | 76.2 | 38 | August 11 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | pSB1AK3 | LuxR repressor | +/- 25 | 41.5 | 36 | August 27 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | pSB1AK3 | LuxR repressor | +/- 50 | 31.7 | 37 | August 27 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | HSL Sender Device | +/- 50 | 57.6 | 16 | August 12 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | HSL Sender Device | +/- 40 | 53.1 | 17 | August 12 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | HSL Sender Device | +/- 50 | 41.4 | 18 | August 27 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | HSL Sender Device | +/- 20 | 31.5 | 19 | August 27 |
| [http://partsregistry.org/Part:BBa_I712022 I712022] | BBa_J52017 (Km, Ap) | T7 polymerase | +/- 40 | 394.1 | 35 | July 31 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 42.5 | 208.2 | 32 | July 29 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 45 | 20.5 | 33 | August 27 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 45 | 63.9 | 34 | August 27 |
| [http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | 3d key+ terminator | +/- 40 | 160.7 | 26 | July 29 |
| [http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | 3d key+ terminator | +/- 46 | 308.3 | 27 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 35 | 117.4 | 23 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 39.5 | 144.8 | 24 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 48 | 129.1 | 25 | July 29 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 46 | 375.1 | 28 | July 29 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 99.2 | 30 | August 27 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 69.2 | 31 | August 27 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 404 | 29 | July 29 |
| [http://partsregistry.org/Part:BBa_J23116 J23116] | BBa_J61002 (Ap) | Constitutive promoter | +/- 48 | 328.8 | 52 | August 08 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | pSB1A2 | LacI regulated promoter | +/- 40 | 69.8 | 42 | July 29 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | pSB1AK3 | LacI regulated promoter | +/- 50 | 30.8 | 40 | August 27 |
| [http://partsregistry.org/Part:BBa_R0040 R0040] | pSB1A2 | tetR repressible promoter | +/- 48 | 37.9 | 46 | August 11 |
| [http://partsregistry.org/Part:BBa_R0062 R0062] | pSB1A2 | LuxR regulated promoter | +/- 40 | 104.1 | 3 | July 31 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | pSB1A2 | OmpF promoter | +/- 40 | 32.0 | 14 | July 23 |
| [http://partsregistry.org/Part:BBa_R1052 R1052] | pSB1K0 | 434cI regulated promoter | +/- 15 | 27.5 | 60 | September 05 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | pSB1A2 | Lactonase | 30 | 155.3 | 54 | July 31 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | pSB1A2 | LuxR repressor/activator | ? | 159.4 | 35 | July 31 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | pSB1A2 | lacI + pL promoter | ? | 68.0 | 39 | July 31 |
| [http://partsregistry.org/Part:BBa_J23066 J23066] | J23006 | ribokey | +/- 45 | 541 | 58 | August 30 |
| [http://partsregistry.org/Part:BBa_P0353 P0353] | pSB1AK3 | cII p22 van memory | +/- 45 | 406.9 | 1 | August 30 |
| [http://partsregistry.org/Part:BBa_P0152 P0152] | pSB1A2 | cI 434 van memory | +/- 44 | 265.4 | 2 | August 30 |
| [http://partsregistry.org/Part:BBa_E0240 E0240] | pSB1A2 | GFP generator | +/- 44 | 281 | 59 | August 30 |
| [http://partsregistry.org/Part:BBa_C0056 C0056] | pSB1A2 | cI represor | +/- 45 | 419.8 | 64 | August 30 |
| [http://partsregistry.org/Part:BBa_C0061 C0061] | pSB1A2 | autoinducer ASL synthetase, LuxI | +/- 45 | 338.6 | 65 | August 30 |
| [http://partsregistry.org/Part:BBa_B0014 B0014] | pSB1AK3 | terminator | +/- 45 | 344.4 | 66 | August 30 |
| [http://partsregistry.org/Part:BBa_E0040 E0040] | pSB1A2 | GFP | +/- 48 | 305.8 | 67 | August 30 |
| [http://partsregistry.org/Part:BBa_R0053 R0053] | pSB1A2 | p22 cII regulated promotor | +/- 25 | 72.1 | 69 | August 30 |
| [http://partsregistry.org/Part:BBa_I714074 I714074] | pSB1A2 | ribokey | +/- 48 | 469 | 68 | August 30 |
| [http://partsregistry.org/Part:BBa_R0040 R0040] | pSB1A2 | tetR repressible promotor | +/- 27 | 74.6 | 45 | September 1 |
| MiniPrep Products: Ligations |
|---|
| Parts | Plasmid | Description | µl left | ng/µl | Box | Date |
|---|---|---|---|---|---|---|
| [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Cell Death | +/- 46 | 124.8 | 1A | August 08 |
| [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Memory | +/- 46 | 276.7 | 5D | September 05 |
| [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Inverter | +/- 46 | 159.1 | 6D | September 05 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240] | pSB1A2 | Ligation for Output | +/- 46 | 281.9 | 7D | September 05 |
| [http://partsregistry.org/Part:BBa_B0014 B0014]+[http://partsregistry.org/Part:BBa_B0033 B0033] | pSB1A2 | Ligation for Cell Death | +/- 46 | 118.1 | 8D | September 05 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066] | J23006 (Ap) | Ligation for Filter (new) | +/- 46 | 421.9 | 9D | September 05 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0033 B0033] | pSB1A2 | Ligation for Memory | +/- 46 | 81.2 | 1E | September 05 |
| [http://partsregistry.org/Part:BBa_R0053 R0053]+[http://partsregistry.org/Part:BBa_P0152 P0152] | pSB1A2 | Ligation for Memory | +/- 46 | 109.5 | 2E | September 05 |
| [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Output | +/- 46 | 151.8 | 5A | August 08 |
| [http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 89.9 | 3A | August 08 |
| [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 94.4 | 4A | August 08 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Input | +/- 40 | 102.5 | 8A | August 25 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Input | +/- 35 | 96.2 | 2B | August 25 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | GFP with LVA tag | +/- 46 | 152.7 | 2A | August 08 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | GFP+LVA | +/- 48 | 257 | 4E | August 30 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB | +/- 48 | 204.6 | 3E | August 30 |
| [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | T7 without UmuD | +/- 48 | 414.8 | 5E | August 30 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB | +/- 48 | 188.4 | 6E | August 30 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB coding region | +/- 46 | 57.8 | 5B | August 28 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB coding region | +/- 46 | 72.1 | 8B | August 28 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB coding region | +/- 46 | 18.5 | 7B | August 28 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB coding region | +/- 46 | 23.3 | 6B | August 28 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for output | +/- 30 | 64.4 | 9A | August 25 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Output | +/- 35 | 93.1 | 1B | August 25 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 35 | 81.3 | 3B | August 27 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 35 | 86.3 | 4B | August 27 |
| [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Inverter | +/- 40 | 226.9 | 1C | September 1 |
| [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Input | +/- 40 | 91.6 | 2C | September 1 |
| [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Input | +/- 40 | 143.8 | 3C | September 1 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Cell Death | +/- 40 | 122.8 | 4C | September 1 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Cell Death | +/- 40 | 119.8 | 5C | September 1 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Output | +/- 40 | 171.5 | 6C | September 1 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Output | +/- 40 | 175.5 | 7C | September 1 |
| [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Reset | +/- 40 | 174.2 | 8C | September 1 |
| [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Reset | +/- 40 | 188.5 | 9C | September 1 |
| [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Filter | +/- 40 | 242.9 | 1D | September 1 |
| [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Filter | +/- 40 | 183.1 | 2D | September 1 |
| [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Inverter | +/- 40 | 56.0 | 3D | September 1 |
| [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Inverter | +/- 40 | 106.3 | 4D | September 1 |
| [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Inverter | +/- 40 | 158.6 | 9B | September 1 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 20 | 150.2 | 6A | August 20 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 20 | 58.3 | 7A | August 20 |
| Restriction Digests |
|---|
| Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
|---|---|---|---|---|---|---|---|---|
| [http://partsregistry.org/Part:BBa_B0015 B0015] | Pst I | Xba I | Insert | 144 | 10 | 4.6 | 3 | August 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3303 | 11 | 11.7 | 1 | July 31 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3303 | 10 | 13.6 | 2 | August 26 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Xba I | Vector | 2092 | 5 | 17.6 | 25 | August 19 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Xba I | Vector | 2092 | 20 | 6.4 | 24 | August 29 |
| [http://partsregistry.org/Part:BBa_B0033 B0033] | EcoR I | Xba I | Vector | 2092 | 20 | 10.9 | 31 | August 27 |
| [http://partsregistry.org/Part:BBa_B0014 B0014] | EcoR I | Spe I | Insert | 110 | 20 | 1.3 | 50 | September 2 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | EcoR I | Spe I | Insert | 677 | 18 | 7.1 | 6 | July 24 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | Spe I | Pst I | Vector | 3192 | 25 | 16.4 | 32 | August 29 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | Spe I | Pst I | Vector | 2853 | 25 | 16.3 | 18 | August 29 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | Spe I | Pst I | Vector | 2724 | 25 | 19.2 | 7 | August 29 |
| [http://partsregistry.org/Part:BBa_K145015 K145015] | Spe I | Pst I | Vector | 2823 | 25 | 18.8 | 33 | August 29 |
| [http://partsregistry.org/Part:BBa_K145151 K145151] | Spe I | Pst I | Vector | 2370 | 25 | 14.5 | 34 | August 29 |
| [http://partsregistry.org/Part:BBa_K145151 K145151] | Spe I | EcoR I | Vector | 2370 | 25 | 25.2 | 49 | August 29 |
| [http://partsregistry.org/Part:BBa_K145001 K145001] | Spe I | Pst I | Vector | 4782 | 20 | 6.2 | 40 | August 29 |
| [http://partsregistry.org/Part:BBa_C0061 C0061] | Spe I | Pst I | Vector | 2680 | 20 | 17.5 | 39 | August 31 |
| [http://partsregistry.org/Part:BBa_C0056 C0056] | Spe I | Pst I | Vector | 2700 | 20 | 19.0 | 36 | August 31 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | EcoR I | Spe I | Insert | 803 | 28 | 5.4 | 17 | August 12 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | EcoR I | Spe I | Insert | 806 | 9 | 8.2 | 16 | August 01 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | EcoR I | Spe I | Insert | 774 | 18 | 24.7 | 11 | August 01 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | EcoR I | Spe I | Insert | 774 | 25 | 8.3 | 12 | August 27 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | EcoR I | Spe I | Insert | 774 | 28 | 5.0 | 10 | August 12 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | EcoR I | Xba I | Vector | 3956 | 26 | 4.5 | 30 | August 19 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3090 | 2 | 12.9 | 20 | July 30 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3090 | 45 | 1.3 | 21 | July 31 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3308 | 25 | 5.1 | 22 | August 07 |
| [http://partsregistry.org/Part:BBa_J23100 J23100] | EcoR I | Spe I | Insert | 52 | 38.1 | 2.6 | 5 | July 24 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | EcoR I | Spe I | Insert | 52 | 21 | 4.2 | 29 | July 29 |
| [http://partsregistry.org/Part:BBa_J23116 J23116] | EcoR I | Spe I | Insert | 52 | +/-20 | 8.1 | 26 | August 12 |
| [http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly) | EcoR I | Spe I | Insert | 2732 | +/-8 | 14.1 | 41 | August 12 |
| [http://partsregistry.org/Part:BBa_K145013 K145013] (aLuxI) | EcoR I | Spe I | Insert | 660 | +/-25 | 20.8 | 53 | September 05 |
| [http://partsregistry.org/Part:BBa_K145150 K145150] (promoter) | EcoR I | Spe I | Insert | 110 | +/-25 | 9.2 | 54 | September 05 |
| [http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly) | EcoR I | Spe I | Insert | 2732 | +/-8 | 24.3 | 48 | August 12 |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] | EcoR I | Spe I | Plasmid | 2079 | 9 | 18.2 | 46 | August 01 |
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] | EcoR I | Spe I | Plasmid | 2056 | 24 | 27.7 | 47 | August 25 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | EcoR I | Spe I | Insert | 72 | 20 | 8.5 | 13 | July 30 |
| [http://partsregistry.org/Part:BBa_E0240 E0240] | EcoR I | Xba I | Vector | 2955 | 28 | 25.2 | 42 | September 1 |
| [http://partsregistry.org/Part:BBa_E0040 E0040] | EcoR I | Spe I | Insert | 720 | 28 | 8.3 | 37 | September 1 |
| [http://partsregistry.org/Part:BBa_C0056 C0056] | EcoR I | Spe I | Insert | 636 | 23 | 10.1 | 35 | September 1 |
| [http://partsregistry.org/Part:BBa_C0061 C0061] | EcoR I | Spe I | Insert | 618 | 28 | 9.0 | 38 | September 1 |
| [http://partsregistry.org/Part:BBa_P0152 P0152] | EcoR I | Xba I | Vector | 2930 | 28 | 23.2 | 43 | September 1 |
| [http://partsregistry.org/Part:BBa_P0353 P0353] | EcoR I | Xba I | Vector | 4000 | 28 | 15.3 | 45 | September 1 |
| [http://partsregistry.org/Part:BBa_J23066 J23066] | EcoR I | XbaI | Vector | 2700 | 28 | 11.4 | 27 | September 1 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3303 | 20 | 12.6 | 4 | September 1 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | EcoR I | Xba I | Vector | 2091 | 28 | 15.6 | 44 | September 1 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | EcoR I | Spe I | Insert | 72 | 20 | 7.5 | 14 | July 30 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | EcoR I | Spe I | Insert | 72 | 25 | 2.1 | 15 | August 27 |
| [http://partsregistry.org/Part:BBa_R0062 R0062] | EcoR I | Spe I | Insert | 55 | 27 | 7.8 | 23 | August 07 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | EcoR I | Spe I | Insert | 125 | 33.4 | 3.8 | 8 | July 24 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | EcoR I | Spe I | Insert | 125 | 25 | 6.1 | 9 | July 29 |
| [http://partsregistry.org/Part:BBa_R0053 R0053] | EcoR I | Spe I | Insert | 70 | 20 | 1.1 | 51 | September 2 |
| [http://partsregistry.org/Part:BBa_R0040 R0040] | EcoR I | Spe I | Insert | 70 | 10 | 0.8 | 52 | September 2 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | EcoR I | Spe I | Insert | 61 | 25 | 3.4 | 28 | July 29 |
| [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 4080 | +/-30 | 32.8 | 81 | August 14 |
| [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 4086 | +/-30 | 14.8 | 80 | August 14 |
| [http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Spe I | Insert | 112 | +/-30 | 3.9 | 79 | August 14 |
| [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Spe I | Insert | 101 | +/-30 | 4.0 | 77 | August 14 |
| [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3113 | +/-20 | 8.3 | 78 | August 14 |
| [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2986 | +/-25 | 17.8 | 67 | September 3 |
| [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2857 | +/-25 | 18.7 | 66 | September 3 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2956 | +/-25 | 20.8 | 65 | September 3 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2505 | +/-25 | 18.1 | 64 | September 3 |
| [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3327 | +/-25 | 18.1 | 63 | September 3 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 71 | +/-28 | 2.5 | 76 | August 25 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 144 | +/-20 | 0.9 | 72 | August 22 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 144 | +/-30 | 2.9 | 71 | August 21 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 90 | +/-28 | 2.6 | 75 | August 25 |
| [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 90 | +/-25 | 3.8 | 62 | September 05 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] | EcoR I | Spe I | Insert | 311 | +/-28 | 3.6 | 74 | August 27 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 144 | +/-28 | 2.4 | 73 | August 25 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022] | EcoR I | Spe I | Insert | 365 | +/-20 | 6.9 | 70 | August 14 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Spe I | Insert | 432 | +/-30 | 4.6 | 68 | August 22 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Spe I | Insert | 432 | +/-30 | 3.7 | 69 | August 22 |
Things stored in freezer in lab (-20°C)
| Miscellaneous |
|---|
| Description | Name | µl left | Concentration | Box | Date |
|---|---|---|---|---|---|
| Primer GFP-LVA | 1084 | 90 | 20 µM | 1 | August 01 |
| Primer GFP-LVA | 1085 | 90 | 20 µM | 2 | August 01 |
| dNTP mix | ATP, GTP, TTP, CTP | 70 | 10 mM | 3 | August 01 |
| Primer GFP-LVA | 1084 | 500 | 100 µM | 4 | August 01 |
| Primer GFP-LVA | 1085 | 500 | 100 µM | 5 | August 01 |
| Primer Test | 1074 | 800 | 100 µM | 6 | August 07 |
| Primer Test | 1075 | 780 | 100 µM | 7 | August 07 |
| Primer T7-UmuD | 1076 | 560 | 100 µM | 8 | August 07 |
| Primer T7-UmuD | 1077 | 477 | 100 µM | 9 | August 07 |
| Primer T7-UmuD | 1078 | 467 | 100 µM | 10 | August 07 |
| Primer T7-UmuD | 1079 | 45 | 100 µM | 11 | August 07 |
| Primer Transduction | 1097 | 627 | 100 µM | 12 | August 07 |
| Primer Transduction | 1098 | 710 | 100 µM | 13 | August 07 |
| Primer Transduction | 1099 | 608 | 100 µM | 14 | August 07 |
| Primer Anti-sense LuxI | 1100 | 522 | 100 µM | 15 | August 07 |
| Primer Anti-sense LuxI | 1101 | 237 | 100 µM | 16 | August 07 |
| Primer Test | 1074 | 100 | 20 µM | 17 | August 07 |
| Primer Test | 1075 | 100 | 20 µM | 18 | August 07 |
| Primer T7-UmuD | 1076 | 100 | 20 µM | 19 | August 07 |
| Primer T7-UmuD | 1077 | 100 | 20 µM | 20 | August 07 |
| Primer T7-UmuD | 1078 | 100 | 20 µM | 21 | August 07 |
| Primer T7-UmuD | 1079 | 100 | 20 µM | 22 | August 07 |
| Primer Transduction | 1097 | 100 | 20 µM | 23 | August 07 |
| Primer Transduction | 1098 | 100 | 20 µM | 24 | August 07 |
| Primer Transduction | 1099 | 100 | 20 µM | 25 | August 07 |
| Primer Anti-sense LuxI | 1100 | 100 | 20 µM | 26 | August 07 |
| Primer Anti-sense LuxI | 1101 | 100 | 20 µM | 27 | August 07 |
| dNTP mix | ATP, GTP, TTP, CTP | 90 | 2 mM | 28 | August 19 |
| Primer ccdB | 1127 | 100 | 100 µM | 29 | August 19 |
| Primer ccdB | 1128 | 100 | 100 µM | 30 | August 19 |
| Primer K145150 | 1129 | 100 | 100 µM | 31 | August 19 |
| Primer K145150 | 1130 | 100 | 100 µM | 32 | August 19 |
| Primer ccdB | 1127 | 90 | 20 µM | 33 | August 19 |
| Primer ccdB | 1128 | 980 | 20 µM | 34 | August 19 |
| Primer K145150 | 1129 | 990 | 1 µM | 35 | August 19 |
| Primer K145150 | 1130 | 990 | 1 µM | 36 | August 19 |
| dNTP mix | ATP, GTP, TTP, CTP | 990 | 2 µM | 37 | August 19 |
