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Team:Chiba/protocol/ligation/dephosphorylation
From 2008.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
#Incubate at 37℃ for 15~30 min. | #Incubate at 37℃ for 15~30 min. | ||
#Incubate at 65℃ for 15 min. (for inactivation by heat treatment) | #Incubate at 65℃ for 15 min. (for inactivation by heat treatment) | ||
| - | #DNA | + | #Purify the DNA with Zymo clean column |
| + | |||
| + | READ:[[Team:Chiba/protocol/DNA_Purification/zymocleam|Zymo cleam]] | ||
Revision as of 03:08, 11 September 2008
dephosphorylation
SAP: Alkaline Phosphatase (Shrimp)
- Mix
DNA fragment 1~10 pmol Shrimp Alkaline Phosphatase (1~5 μ l) 1~5 U 10X SAP Buffer 5 μ l Sterilized distilled water up to 50 μ l
- Incubate at 37℃ for 15~30 min.
- Incubate at 65℃ for 15 min. (for inactivation by heat treatment)
- Purify the DNA with Zymo clean column
READ:Zymo cleam
