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Team:KULeuven/Data/GFP
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===Materials and methods=== | ===Materials and methods=== | ||
Electrocompetent Top10 cells were electroporated with the | Electrocompetent Top10 cells were electroporated with the | ||
| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K145015 K145015] part in a pSB1A2 vector. These were plated out on LB plates with ampicillin and grown overnight (37°). From this, a liquid culture supplied with | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K145015 K145015] part in a pSB1A2 vector. These were plated out on LB plates with ampicillin and grown overnight (37°). From this, a liquid culture supplied with 100 µg ampicillin / ml was prepared and also grown overnight (37°C). These cells were inoculated in fresh LB medium (100 µg ampicillin / ml) in the morning and grown at 37°C for 3.5h. They were then washed in preheated (37°C) ABT minimal medium and resuspended in the same medium and the same amount of medium. After that, cells were whitdrawn at different times and FACS analysis on distribution of fluorescence was done. |
Revision as of 14:29, 17 September 2008
GFP with LVA-tag
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K145015 Parts Registry:K145015]
Materials and methods
Electrocompetent Top10 cells were electroporated with the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K145015 K145015] part in a pSB1A2 vector. These were plated out on LB plates with ampicillin and grown overnight (37°). From this, a liquid culture supplied with 100 µg ampicillin / ml was prepared and also grown overnight (37°C). These cells were inoculated in fresh LB medium (100 µg ampicillin / ml) in the morning and grown at 37°C for 3.5h. They were then washed in preheated (37°C) ABT minimal medium and resuspended in the same medium and the same amount of medium. After that, cells were whitdrawn at different times and FACS analysis on distribution of fluorescence was done.
