Revision as of 22:11, 3 October 2008

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Things we did today

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. One microliter of PCR reaction were loaded into each well.

Construct	Colonies
nir+rbs+pilA	95
nir+rbs+GFP	10
nir+rbs+slr1+GFPf	42
rbs+slr1+GFPf	2
rbs+GFP+tt	3
slr1+GFPf+tt	2
pSB1A3 (to self, negative control)	330
no DNA	0

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ===Verification of transformants===
 :<strong> Grace</strong>
-[[Image:092208colonypcr.png|right|thumb|500px|EtBr stained 2% agarose gel ran at 95V for 1 hour. One microliter of PCR reaction were loaded into each well.]]
+[[Image:092208colonypcr.png|right|thumb|600px|EtBr stained 2% agarose gel ran at 95V for 1 hour. One microliter of PCR reaction were loaded into each well.]]
 {|class=wikitable align=center border=1
 !Construct