Revision as of 18:22, 14 June 2008

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Things we did today

Krystle

**Title**
Antibiotic	Cell type	Plasmid
Amp₁₀₀	StrataClone supercompetent cells	pUC18
	DH5α	GFP
	DH5α	tet+GFP
	DH5α	RBS (BBa_B0034)
Kan₅₀	HB101	RK1
Sm_2.5 + Sp _2.5	DH5α	pRL1383a

Grace

Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
Made SOB for new inoculation.
Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5α. Incubated overnight at 27C with shaking (150 rpm).

Grace

Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
Made BG-11 plates for retest next week.

Grace

We need to make more BG-ll 50x stock solution and TES 50x stock solution
Kelly from SC lab will be in 12pm-3pm. We should retrieve our triparental conjugation plates then.
Grace, Krystle, and Norman will come in at 9am tomorrow to plate triparental conjugates, make competent cells, and glycerol stock purified Biobrick colonies.

E. coli homes. Enough said.

Krystle & Grace confusing Norman, again. Two halves of the same person?

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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 = Quote of the Day =
-[[Image:DSC00746.JPG |thumb|left|225px| ''E. coli'' homes. Enough said.]]
+[[Image:DSC00746.JPG |thumb|left|225px| ''E. coli'' homes. Enough said.]][[Image:DSC00747.JPG|thumb|right|225px|Krystle & Grace confusing Norman, again. Two halves of the same person?]]
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