Team:Chiba/Calendar-Home/29 August 2008

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Revision as of 20:27, 29 October 2008

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28 August 2008 <|> 30 August 2008

Laboratory work

Team:Input

no work

Team:Communication

--->Digestion Test

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
[http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2007)
[http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2007)
[http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2006)
[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2007)
[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2006)
[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2007)
[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2006)
[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2007)
[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2006)

Sample No.	S1~10	W1	W2~10
Sample DNA	1	4	2
PstI	-	0.075	0.1
EcoRI	0.1	0.075	0.1
Buffer 3	1	0.75	1
BSA	-	0.75	1
dH₂O	7.9	4.35	5.8
TOTAL	10μl	10μl	10μl

--->Gel Check

Sample No.	1~10	S1~S10,W1~W10
Sample DNA	1	10
Loading Dye	1	2
TOTAL	6μl	12μl

From left;

1~5,S1,S5,W1~W5

From left;

6~10,S6~S10,W6~W10

(27/8)--->Gel Check

Sample DNA	0.5
Loading Dye	1
dH₂O	4.5
TOTAL	6μL

[http://partregistry.org/Part:R0010 R0010] -> OK

Digestion

[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2006)

Sample No.	1~3	4
Sample DNA	100ng/μL×20	200ng/μL×30
PstI	2	2
XbaI	2	-
SpeI	-	2
Buffer 2	-	5
Buffer 3	5	-
BSA	5	5
dH₂O	16	6
TOTAL	50μl	50μl

--->Gel Check

Sample No.	1~3	4
Sample DNA	10	3
Loading Dye	2	2
dH₂O	-	7
TOTAL	12μl	12μl

insert[http://partsregistry.org/Part:BBa_I9026 BBa_I9026] : 685bp -> None

insert[http://partsregistry.org/Part:BBa_I9026 BBa_I9026] : 745bp -> None

insert[http://partsregistry.org/Part:BBa_I9026 BBa_S03154 BBa_S03154] : 685bp -> None

vector[http://partsregistry.org/Part:BBa_I9026 BBa_R0010 BBa_R0010] : 2079bp -> OK

--->Gel Extract

--->Zymo Clean

->15μL

--->SAP

Sample No.	4
Sample DNA	15
SAP	2
SAP Buffer	3
dH₂O	10
TOTAL	30μL

--->Zymo Clean

--->Suspension! (We got information, by the team-output, to the effect that the density of the sample is too low.)

Team:Output

TIME RESPONCE(solid)

@@ Line 32: / Line 32: @@
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI</td>
 <td>-</td><td>0.075</td><td>0.1</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRI</td>
 <td>0.1</td><td>0.075</td><td>0.1</td>
 </tr>
@@ Line 53: / Line 53: @@
 <tr>
 <td>TOTAL</td>
-<td>10</td><td>10</td><td>10</td>
+<td>10μl</td><td>10μl</td><td>10μl</td>
 </tr>
 </table>
@@ Line 77: / Line 77: @@
 <tr>
 <td>TOTAL</td>
-<td>6</td><td>12</td></tr>
+<td>6μl</td><td>12μl</td></tr>
 </table>
 :From left;<br>
@@ Line 113: / Line 113: @@
 </table>
-::R0010 -> OK
+::[http://partregistry.org/Part:R0010 R0010] -> OK
 |}
@@ Line 134: / Line 134: @@
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI</td>
 <td>2</td><td>2</td>
 </tr>
 <tr>
-<td>XbaⅠ</td>
+<td>XbaI</td>
 <td>2</td><td>-</td>
 </tr>
 <tr>
-<td>SpeⅠ</td>
+<td>SpeI</td>
 <td>-</td><td>2</td></tr>
 <tr>
@@ Line 160: / Line 160: @@
 <tr>
 <td>TOTAL</td>
-<td>50</td><td>50</td>
+<td>50μl</td><td>50μl</td>
 </tr>
 </table>
@@ Line 187: / Line 187: @@
 <tr>
 <td>TOTAL</td>
-<td>12</td><td>12</td></tr>
+<td>12μl</td><td>12μl</td></tr>
 </table>
-:insert-I9026 : 685bp -> None<br>
+:insert[http://partsregistry.org/Part:BBa_I9026 BBa_I9026] : 685bp -> None<br>
 |}
 {|align="justify"
 |[[Image:Chiba-0829-5.JPG]]
 |
-:insert-I9030 : 745bp -> None<br>
+:insert[http://partsregistry.org/Part:BBa_I9026 BBa_I9026]  : 745bp -> None<br>
 |}
 {|align="justify"
 |[[Image:Chiba-0829-6.JPG]]
 |
-:insert-S03154 : 685bp -> None<br>
+:insert[http://partsregistry.org/Part:BBa_I9026 BBa_S03154 BBa_S03154] : 685bp -> None<br>
 |}
 {|align="justify"
 |[[Image:Chiba-0829-7.JPG]]
 |
-:vector-R0010 : 2079bp -> OK<br>
+:vector[http://partsregistry.org/Part:BBa_I9026 BBa_R0010 BBa_R0010] : 2079bp -> OK<br>
 |}
@@ Line 243: / Line 243: @@
 ::--->Suspension! (We got information, by the team-output, to the effect that the density of the sample is too low.)
 ===Team:Output===
 [[Team:Chiba/jk/γ/trs|TIME RESPONCE(solid)]]