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Alberta NINT/27 May 2008
From 2008.igem.org
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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lab work (SD): | lab work (SD): | ||
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Transformed XL1-B cells and plated on LB+Amp100 plates. | Transformed XL1-B cells and plated on LB+Amp100 plates. | ||
Created 2 O/Ns as well. | Created 2 O/Ns as well. | ||
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| + | [[Alberta_NINT/28_May_2008 | Next entry >]] | ||
Revision as of 21:27, 19 June 2008
lab work (SD):
DNA digest of K102000 with SpeI + PstI. Incubated at 37 C for 1 hour.
Run on 2% agarose gel. ~3 kbp fragment expected.
Ligated K102000 with E0433 and K102000 with null_output.
lab work (JD):
Recovered Part BBA_R011 from iGEM package
Transformed XL1-B cells and plated on LB+Amp100 plates.
Created 2 O/Ns as well.
