Team:Princeton/Experiments

From 2008.igem.org

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'''Lab Protocols'''
'''Lab Protocols'''
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1. [[Primer Design]]
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1. Primer Design
2. [[PCR Amplification]]
2. [[PCR Amplification]]

Revision as of 23:45, 28 October 2008

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PRINCETON IGEM 2008

Home Project Overview Project Details Experiments Results Notebook
Parts Submitted to the Registry Modeling The Team Gallery




Bio-brick Creation

Lab Protocols

1. Primer Design

2. PCR Amplification

3. Run Gel/ Gel extraction

4. PCR SOEing

5. Digestion

6. CIP Treatment

7. PCR Purification

8. Ligate DNA

9. Transformation and Plating

10. Extract DNA (Miniprep)

11. Restriction Map/ Restriction Digest

12. Re-transform with selected plasmid

13. Extract DNA (Maxiprep or Midiprep)

14. Sequence

Bio-Brick Verification

Cell Culture Process

1. Lentivirus Production

2. Lentivirus Harvesting

3. Infection


Also,

1. Transfection (nonviral)

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