Team:ESBS-Strasbourg/Protocols
From 2008.igem.org
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==Solutions and buffers== | ==Solutions and buffers== | ||
- | * | + | *[http://openwetware.org/wiki/SOB SOB for the preparation of competent cells] <br> |
- | * | + | *[http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes] <br> |
- | * | + | *for the do it yourself MiniPrep [[Team:ESBS-Strasbourg/TENS|TENS for the do it yourself MiniPrep]] <br> |
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Revision as of 23:58, 28 October 2008
E.Coli
- Preparation of competent cells (Barbara's Protocol)
- [http://openwetware.org/wiki/TOP10_chemically_competent_cells Preparation of TOP10 chemically competent cells (iGEM)]
- Transformation of competent cells (Barbara's Protocol)
- Heat shock transformation
Cloning |
Solutions and buffers
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Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]