Team:Paris/Modeling/f0

From 2008.igem.org

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[[Image:eqF0.jpg]]
[[Image:eqF0.jpg]]
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{|border="1" style="text-align: center"
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{{Paris/Toggle|Prior for Characterization|Team:Paris/Modeling/More_f0}}  
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|param
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</div>
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|signification
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|unit
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|value
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|comments
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|[exprJ23101]
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|expression rate of <br> J23101 '''with RBS E0032'''
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|nM.min<sup>-1</sup>
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|γ<sub>GFP</sub>
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|dilution-degradation rate <br> of GFP(mut3b)
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|min<sup>-1</sup>
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|0.0198
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|Only dilution :<br>Time Cell Division : 35 min.
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|[GFP]
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|GFP concentration at steady-state
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|nM
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|-
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|(''fluorescence'')
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|value of the observed fluorescence
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|au
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|mean value of ~20 measures
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|''conversion''
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|conversion ratio between <br> fluorescence and concentration
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|nM.au<sup>-1</sup>
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|(1/79.429)
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</center>
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<center>That will directly give us &#131;0</center>
<center>That will directly give us &#131;0</center>

Revision as of 08:46, 29 October 2008

Method & Algorithm : ƒ0


Specific Plasmid Characterisation for ƒ0
In the exponential phase of growth, at the steady state, we have

EqF0.jpg

↓ Prior for Characterization ↑


param signification unit value comments
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration
↓ gives ↓ 		nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓ 		min-1 0.0198 Only dilution :
Time Cell Division : 35 min.
ƒ0 activity of
J23101 with RBS E0032 		nM.min-1


That will directly give us ƒ0
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