Team:Waterloo/Notebook/Oligos

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Revision as of 02:38, 30 October 2008

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Description	Oligo Name	Sequence	Length
BioBrick sequencing/colPCR primer (fwd)	iGEMvf2	TGCCACCTGACGTCTAAGAA	20
BioBrick sequencing/colPCR primer (rev)	iGEMvr	ATTACCGCCTTTGAGTGAGC	20
Primer for Lac promoter (fwd)	PLacF	TTATGCTTCCGGCTCGTATG	20
Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2)	iGEMpre+rbs	TTTCTCCTCTTTCTCTAGATGCGG	24
Primer for {BioBrick Prefix}-{B0034} (fwd; for use with iGEMvr)	PrefixRBS-F	GGCCGCTTCTAGAGAAAGAGG	21
Gene 5 ORF from T7 phage (fwd; Prefix adapter)	T7gene5-F	CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA	46
Gene 5 ORF from T7 phage (rev; Suffix adapter)	T7gene5-R	CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG	51
Gene 6 ORF from T7 phage (fwd; Prefix adapter)	T7gene6-F	CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA	46
Gene 6 ORF from T7 phage (rev; Suffix adapter)	T7gene6-R	CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG	43
PmeIR ORF (fwd)	PmeIR-F	ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG	50
PmeIR ORF (rev)	PmelR-R	CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG	52
pRecA oligo (sense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR)	recAF	AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA	71
pRecA oligo (antisense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR)	recAR	CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG	71
{B0034}-{T7gene6 ORF} (fwd; for adding {Prefix-RBS} onto the gene 6 ORF by PCR)	RBST7g6-F	GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC	61
T7gene6 ORF (rev)	T7gs-R	CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC	48
	T7g6-RXO-RBS	CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC	53
	RBSpmeI-F	GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG	61
	RBSpmeI-FXO	TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG	48
	pmeI-R	CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG	45
	R0011-F	GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC	38
	R0011RBS-RXO	CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC	47
	RBSC0051-F	GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC	59
	RBSC0051-FXO	TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC	46
	C0051-R	CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG	40
	C0051-RXO	GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG	36
	B0015-FXO	TAATAATACTAGAGCCAGGCATCAAATAAAACG	33
	B0015-R	CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC	39
	RFP-RBS-F	GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG	58
	RFP-RXO	GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG	34
	Plac-RBS-F	GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACATACTAGAGAAAGAGGAGAAATACTAG	103

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 {| border="1" cellpadding="2"
-!width="500"|Description
+! Description !! Oligo Name !! Sequence !! Length
-!width="500"|Oligo Name
-!width="500"|Sequence
-!width="500"|Length
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 |BioBrick sequencing/colPCR primer (fwd)  || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20