Team:Paris/Modeling/f0

From 2008.igem.org

(Difference between revisions)
Line 12: Line 12:
<div style="text-align: center">
<div style="text-align: center">
-
{{Paris/Toggle|Table|Team:Paris/Modeling/More_f0}}  
+
{{Paris/Toggle|Table of Values|Team:Paris/Modeling/More_f0}}  
</div>
</div>

Revision as of 01:47, 30 October 2008

Method & Algorithm : ƒ0


The determination of ƒ0 is really simple :

Specific Plasmid Characterisation for ƒ0

According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state, we have

EqF0.jpg

↓ Table of Values ↑


param signification unit value comments
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration
↓ gives ↓ 		nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓ 		min-1 0.0198 Only dilution :
Time Cell Division : 35 min.
ƒ0 activity of
J23101 with RBS E0032 		nM.min-1


That will directly give us ƒ0



<Back - to "Implementation" |
<Back - to "Protocol_Of_Characterization" |

Retrieved from "http://2008.igem.org/Team:Paris/Modeling/f0"