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Team:NTU-Singapore/Notebook/30 June 2008
From 2008.igem.org
(Difference between revisions)
(New page: *Chin Chong **Stock check of items purchased so far and shifted them into new lab with Darius's help **Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed th...) |
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| - | + | Chin Chong | |
| - | + | *Stock check of items purchased so far and shifted them into new lab with Darius's help | |
| - | + | *Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks | |
| - | + | *Purchased items from SBS general store | |
| - | + | *Did inoculation of colonies from Top cells containing: | |
#LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004], | #LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004], | ||
#Standard promoter [http://partsregistry.org/Part:BBa_J23101 BBa_J23101] | #Standard promoter [http://partsregistry.org/Part:BBa_J23101 BBa_J23101] | ||
| Line 9: | Line 9: | ||
#Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and | #Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and | ||
#Strong [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] promoters | #Strong [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] promoters | ||
| - | + | *Incubate cells in 5ml of LB broth containing the respective anitbiotics and allow them to grow overnight | |
| + | **This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead of LB broth | ||
| + | **M9 medium with glycerol will be introduced in the following day after the cells have been allowed to grow overnight in LB | ||
Revision as of 13:47, 30 June 2008
Chin Chong
- Stock check of items purchased so far and shifted them into new lab with Darius's help
- Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks
- Purchased items from SBS general store
- Did inoculation of colonies from Top cells containing:
- LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004],
- Standard promoter [http://partsregistry.org/Part:BBa_J23101 BBa_J23101]
- GFP reporter devices with Weak [http://partsregistry.org/Part:BBa_J23150 BBa_J23150],
- Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and
- Strong [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] promoters
- Incubate cells in 5ml of LB broth containing the respective anitbiotics and allow them to grow overnight
- This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead of LB broth
- M9 medium with glycerol will be introduced in the following day after the cells have been allowed to grow overnight in LB
