Team:Paris/Construction

From 2008.igem.org

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|- style="background: #dddddd;"   
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| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)
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|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFLiL is ECFP.
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|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFliL(+ RBS WT)-GFP
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|align="center"|  
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|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA+)
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|align="center"|  
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|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag.
|align="center"| [[Team:Paris/August 22|August 22]]
|align="center"| [[Team:Paris/August 22|August 22]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> pFlgA(+ RBS WT) - YFP Tripart (LVA-)
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|align="center"|  
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|align=|Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. 
|align="center"| [[Team:Paris/August 22|August 22]]
|align="center"| [[Team:Paris/August 22|August 22]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)
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|align="center"|  
+
|align="center"| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag.
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlgA(+ RBS WT)-GFP
-
|align="center"|  
+
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP.
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-mRFP tripart (+LVA)
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|align="center"|  
+
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag.
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]]<br>pFlhB(+ RBS WT)-GFP
-
|align="center"|  
+
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP.
|align="center"| [[Team:Paris/October 26|October 26]]
|align="center"| [[Team:Paris/October 26|October 26]]
|-
|-

Revision as of 02:47, 30 October 2008

FIFO
Construction Description Validation Date
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (LVA+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. August 22
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA-) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. August 22
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT)-YFP tripart (AAV+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. October 24
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-mRFP tripart (+LVA) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. October 26
OSCILLATION
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFliL(+ RBS WT)-EnvZ* 		October 26
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(+ RBS WT)-EnvZ*-GFP generator 		October 29
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlhB(+ RBS WT)-EnvZ* 		October 24
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-EnvZ*-GFP generator 		October 29
Icon regulatory.pngIcon coding.png
araC/pBad-EnvZ* 		October 27
SYNCHRONISATION
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart 		August 1
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP 		August 1
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-rbs-lasR-dble ter 		August 20
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
Strong promoter-rbs-lasR-dble ter 		August 20
Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA) 		August 13
Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + dbl ter 		August 19
Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator 		August 1
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - GFP tripart 		August 13
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP 		August 13
NEW GENE AND PROMOTER BIOBRICKS
Part icon regulatory.png
pFliA 		October 04
Part icon regulatory.png
pFliL (+RBS WT) 		October 10
Part icon regulatory.png
pFlgA (+RBS WT) 		October 10
Part icon regulatory.png
pFlgB (+RBS WT) 		October 10
Part icon regulatory.png
pFlhB (+RBS WT) 		October 10
Icon coding.png
EnvZ* 		September 04
Icon coding.png
FliA 		September 04
Icon coding.png
FlhDC 		August 13
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