Team:Paris/Construction

From 2008.igem.org

(Difference between revisions)
Line 26: Line 26:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlgA(+ RBS WT)-YFP tripart (AAV+)
-
|align="center"| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag.  
+
|align=| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag.  
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 45: Line 45:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 26|October 26]]
|align="center"| [[Team:Paris/October 26|October 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+  RBS WT)-EnvZ*-GFP generator
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+  RBS WT)-EnvZ*-GFP generator
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 29|October 29]]
|align="center"| [[Team:Paris/October 29|October 29]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 29|October 29]]
|align="center"| [[Team:Paris/October 29|October 29]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 27|October 27]]
|align="center"| [[Team:Paris/October 27|October 27]]
|-
|-
Line 68: Line 68:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 1|August 1]]
|align="center"| [[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 1|August 1]]
|align="center"| [[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 20|August 20]]
|align="center"| [[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 20|August 20]]
|align="center"| [[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 13|August 13]]
|align="center"| [[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 19|August 19]]
|align="center"| [[Team:Paris/August 19|August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 1|August 1]]
|align="center"| [[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 13|August 13]]
|align="center"| [[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP  
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP  
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 13|August 13]]
|align="center"| [[Team:Paris/August 13|August 13]]
|-
|-
Line 107: Line 107:
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 04|October 04]]
|align="center"| [[Team:Paris/October 04|October 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliL (+RBS WT)
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 10|October 10]]
|align="center"| [[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgA (+RBS WT)
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 10|October 10]]
|align="center"| [[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlgB (+RBS WT)
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 10|October 10]]
|align="center"| [[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFlhB (+RBS WT)
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/October 10|October 10]]
|align="center"| [[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>EnvZ*
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/September 04|September 04]]
|align="center"| [[Team:Paris/September 04|September 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FliA
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/September 04|September 04]]
|align="center"| [[Team:Paris/September 04|September 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]]<br>FlhDC
-
|align="center"|  
+
|align=|  
|align="center"| [[Team:Paris/August 13|August 13]]
|align="center"| [[Team:Paris/August 13|August 13]]
|}
|}

Revision as of 02:49, 30 October 2008

FIFO
Construction Description Validation Date
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (LVA+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. August 22
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA-) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. August 22
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT)-YFP tripart (AAV+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. October 24
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-mRFP tripart (+LVA) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. October 26
OSCILLATION
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFliL(+ RBS WT)-EnvZ* 		October 26
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(+ RBS WT)-EnvZ*-GFP generator 		October 29
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlhB(+ RBS WT)-EnvZ* 		October 24
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-EnvZ*-GFP generator 		October 29
Icon regulatory.pngIcon coding.png
araC/pBad-EnvZ* 		October 27
SYNCHRONISATION
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart 		August 1
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP 		August 1
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-rbs-lasR-dble ter 		August 20
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
Strong promoter-rbs-lasR-dble ter 		August 20
Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA) 		August 13
Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + dbl ter 		August 19
Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator 		August 1
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - GFP tripart 		August 13
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP 		August 13
NEW GENE AND PROMOTER BIOBRICKS
Part icon regulatory.png
pFliA 		October 04
Part icon regulatory.png
pFliL (+RBS WT) 		October 10
Part icon regulatory.png
pFlgA (+RBS WT) 		October 10
Part icon regulatory.png
pFlgB (+RBS WT) 		October 10
Part icon regulatory.png
pFlhB (+RBS WT) 		October 10
Icon coding.png
EnvZ* 		September 04
Icon coding.png
FliA 		September 04
Icon coding.png
FlhDC 		August 13
Retrieved from "http://2008.igem.org/Team:Paris/Construction"