Team:Hawaii

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Revision as of 03:13, 30 October 2008

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The project

The main goal of the Hawaii team is to make a "toolkit" for cyanobacteria, which are photosynthetic bacteria that can convert carbon dioxide to sugars using the same machinery as plants. Cyanobacteria thus hold promise as producers of biofuels and other bioproducts.

We are constructed a number of tools to more easily and effectively engineer cyanobacteria, including

1) a BioBrick switch that turns on a downstream gene when nitrate is added,

2) a second BioBrick that mediates protein export from the cell into the surrounding medium and

3) a naturally occurring shuttle vector to BioBrick format to allow easy transfer of existing and new BioBricks into cyanobacteria.

Parts	Help	Links/Resources
[http://partsregistry.org/cgi/partsdb/search.cgi Parts Search] [http://partsregistry.org/assembly/libraries.cgi?id=15 Parts Kit Content (Spring 2008)] [http://partsregistry.org/Help:IGEM_08_DNA_distribution Parts Kit Instructions] Parts We Submitted	[http://partsregistry.org/wiki/index.php/Help:Contents BioBrick Help] Wiki Help	Protocols iGEM 2008 Homepage [http://partsregistry.org Parts Registry Homepage]

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ==The project==
-===A BioBrick toolkit for cyanobacteria===
+:The main goal of the Hawaii team is to make a "toolkit" for cyanobacteria, which are photosynthetic bacteria that can convert carbon dioxide to sugars using the same machinery  as plants.  Cyanobacteria thus hold promise as producers of biofuels and other bioproducts.
-:We aim to extend the current BioBrick registry to a greater range of organisms, including cyanobacteria. Cyanobacteria are studied for their ability to produce useful compounds, including biofuels and biopolymers. These "little green factories" require only salts, light, water, and carbon dioxide for photoautotrophic growth. A cyanobacterial "toolkit" would enhance our ability to utilize this system.
+:We are constructed a number of tools to more easily and effectively engineer cyanobacteria, including
+:::1) a BioBrick switch that turns on a downstream gene when nitrate is added,
+:::2) a second BioBrick that mediates protein export from the cell into the surrounding medium and
+:::3) a naturally occurring shuttle vector to BioBrick format to allow easy transfer of existing and new BioBricks into cyanobacteria.
-::We designed:
-:::1) mobilizable broad-host range BioBrick vectors derived from RSF1010, <br>
-:::2) a cassette for protein secretion from ''Synechocystis'' sp. PCC 6803, and <br>
-:::3) a nitrate-inducible cyanobacterial promoter BioBrick.
-:Our toolkit was designed for conjugative gene transfer from ''Escherichia coli'' to ''Synechocystis'' to achieve the controlled production and recovery or bioproducts, demonstrable by induced secretion of green fluorescent protein. Though our parts were targeted for work in cyanobacteria, they may be compatible with other Gram-negative systems including ''Agrobacterium'', which is capable of plant transformation.