Team:Chiba/Calendar-Home/19 August 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Communication)
 
Line 24: Line 24:
</tr>
</tr>
<tr>
<tr>
-
<td>dNTP mix</td>
+
<td>dNTP mix(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Foward Primer</td>
+
<td>Foward Primer(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Reverse Primer</td>
+
<td>Reverse Primer(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA polymerase VENT</td>
+
<td>DNA polymerase VENT(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Thermopol Buffer</td>
+
<td>Thermopol Buffer(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>28</td>
<td>28</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>50μL</td>
+
<td>50</td>
</tr>
</tr>
</table>
</table>
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</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td>
<td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>7</td>
<td>7</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>12μL</td>
+
<td>12</td>
</tr>
</tr>
</table>
</table>

Latest revision as of 05:55, 30 October 2008

>Home | Notebook

18 August 2008 <|> 20 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

PCR
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154 BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154 BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control


DNA Template 1
dNTP mix(μL) 10
Foward Primer(μL) 5
Reverse Primer(μL) 5
DNA polymerase VENT(μL) 1
Thermopol Buffer(μL) 5
dH2O(μL) 28
TOTAL(μL) 50


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


--->Gel Check
Chiba-0819.JPG
Sample DNA 3
Loading Dye(μL) 2
dH2O(μL) 7
TOTAL(μL) 12
From left;
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) ->None
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) ->None
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) ->None
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) ->None
  • [http://partsregistry.org/Part:BBa_S3154 BBa_S3154](2007) ->None
  • [http://partsregistry.org/Part:BBa_S3154 BBa_S3154](2006) ->None
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control ->OK

Team:Output

no work

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