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Virginia/8 July 2008
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(New page: ==Goals== *Prepare overnight broth of successful single colony of Promoter + RBS transformation *Plate Screening plasmid (psb1a10) when it arrives from iGem *Try again with ligation of RFP...)
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(New page: ==Goals== *Prepare overnight broth of successful single colony of Promoter + RBS transformation *Plate Screening plasmid (psb1a10) when it arrives from iGem *Try again with ligation of RFP...)
Newer edit →
Revision as of 14:40, 8 July 2008
Goals
- Prepare overnight broth of successful single colony of Promoter + RBS transformation
- Plate Screening plasmid (psb1a10) when it arrives from iGem
- Try again with ligation of RFP and GFP enzyme cut DNA
- Transform this ligation and pray that it grows in the incubator
- Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
- Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon
Notes
- Maxiprepped bba_B0015 and bba_B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA
