Alberta NINT/27 May 2008

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             Transformed XL1-B cells and plated on LB+Amp100 plates.   
             Transformed XL1-B cells and plated on LB+Amp100 plates.   
             Created 2 O/Ns as well.
             Created 2 O/Ns as well.
 +
 +
lab work (WM):
 +
 +
            Prepare BBa_K102010 fragments for ligation.
 +
            K102010 is AraC::PromBad::TA0In
 +
            AraC::PromBad comes is in BBa_I0500/SpeI+PstI (5.6kb fragment, including plasmid)
 +
            TA0In (200bp) was synthesized commercially and placed in a vector.
 +
            Gel-purify BBa_I0500/SpeI+PstI and TA0In?XbaI+PstI fragments from 2% agarose gel.
 +
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Revision as of 17:16, 8 July 2008

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lab work (SD): 

           DNA digest of K102000 with SpeI + PstI.  Incubated at 37 C for 1 hour.  
           Run on 2% agarose gel.  ~3 kbp fragment expected.  
           Ligated K102000 with E0433 and K102000 with null_output.

lab work (JD): 

            Recovered Part BBA_R011 from iGEM package 
            Transformed XL1-B cells and plated on LB+Amp100 plates.  
            Created 2 O/Ns as well.

lab work (WM): 

            Prepare BBa_K102010 fragments for ligation.
            K102010 is AraC::PromBad::TA0In
            AraC::PromBad comes is in BBa_I0500/SpeI+PstI (5.6kb fragment, including plasmid)
            TA0In (200bp) was synthesized commercially and placed in a vector.
            Gel-purify BBa_I0500/SpeI+PstI and TA0In?XbaI+PstI fragments from 2% agarose gel.


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