Team:Rice University/Notebook/13 July 2008

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(New page: =Sunday, July 13th= *Taylor Stevenson *#Innoculated 100mL of LB with 1.0mL of O/N cultures and grew at 37*C (for all strains excluding the VCS257 containing the temperature sensitive phage...)
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*#Cells were made electrocompetent following the procedure outlined [a;lsdkjf|here].
*#Cells were made electrocompetent following the procedure outlined [a;lsdkjf|here].
*#Procedure resulted in 10x40uL stocks of each of the 5 strains.
*#Procedure resulted in 10x40uL stocks of each of the 5 strains.
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Rice_University|Home]]
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!align="center"|[[Team:Rice_University/Team|The Team]]
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!align="center"|[[Team:Rice_University/Project|The Project]]
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!align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Rice_University/Modeling|Modeling]]
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!align="center"|[[Team:Rice_University/Notebook|Notebook]]
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Revision as of 19:21, 16 July 2008

Sunday, July 13th

  • Taylor Stevenson
    1. Innoculated 100mL of LB with 1.0mL of O/N cultures and grew at 37*C (for all strains excluding the VCS257 containing the temperature sensitive phage. All 5.0mL of this O/N were used to innoculate the 100mL of LB. The Resulting culture was grown at 30*C).
    2. All strains were grown to OD600nm of approximately 0.8 (Note: the 7524 strain never reached an appropriate optical density, however, I continued the prep with the slightly reduced titer).
    3. Cells were made electrocompetent following the procedure outlined [a;lsdkjf|here].
    4. Procedure resulted in 10x40uL stocks of each of the 5 strains.






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