Latest revision as of 19:23, 16 July 2008

Sunday, July 13th

Taylor Stevenson
1. Innoculated 100mL of LB with 1.0mL of O/N cultures and grew at 37*C (for all strains excluding the VCS257 containing the temperature sensitive phage. All 5.0mL of this O/N were used to innoculate the 100mL of LB. The Resulting culture was grown at 30*C).
2. All strains were grown to OD600nm of approximately 0.8 (Note: the 7524 strain never reached an appropriate optical density, however, I continued the prep with the slightly reduced titer).
3. Cells were made electrocompetent following the procedure outlined here.
4. Procedure resulted in 10x40uL stocks of each of the 5 strains.

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 *#Innoculated 100mL of LB with 1.0mL of O/N cultures and grew at 37*C (for all strains excluding the VCS257 containing the temperature sensitive phage.  All 5.0mL of this O/N were used to innoculate the 100mL of LB.  The Resulting culture was grown at 30*C).
 *#All strains were grown to OD600nm of approximately 0.8 (Note: the 7524 strain never reached an appropriate optical density, however, I continued the prep with the slightly reduced titer).
-*#Cells were made electrocompetent following the procedure outlined [a;lsdkjf|here].
+*#Cells were made electrocompetent following the procedure outlined [[Team:Rice University/Electroporation|here]].
 *#Procedure resulted in 10x40uL stocks of each of the 5 strains.