Team:ESBS-Strasbourg/Protocols

From 2008.igem.org

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(Solutions and buffers)
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*'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br>
*'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br>
*'''SOC''' for bacterial transformation purposes [http://openwetware.org/wiki/SOC SOC] <br>
*'''SOC''' for bacterial transformation purposes [http://openwetware.org/wiki/SOC SOC] <br>
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*'''TENS''' for the do it yourself MiniPrep [http://openwetware.org/wiki/TENS TENS] <br>
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Revision as of 09:32, 17 July 2008

<= back

E.Coli

  • Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]
  • Transformation of competent cells (Mariels/Barbaras Protocol) Transformation


Cloning

Solutions and buffers

  • SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]
  • SOC for bacterial transformation purposes [http://openwetware.org/wiki/SOC SOC]
  • TENS for the do it yourself MiniPrep [http://openwetware.org/wiki/TENS TENS]


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