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Virginia/17 July 2008
From 2008.igem.org
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*Grow E0240 in liquid broth from originally growing plate that came from the new DNA spot in the most recent newsletter | *Grow E0240 in liquid broth from originally growing plate that came from the new DNA spot in the most recent newsletter | ||
**we need to prove that this gene will glow green before we try to make it fluoresce in an assembly | **we need to prove that this gene will glow green before we try to make it fluoresce in an assembly | ||
| - | *Use overnight broth of R0010 + A4 from night before to miniprep and grow in a LAC liquid culture | + | *Use overnight broth of A6(R0010 + A4) from night before to miniprep and grow in a LAC liquid culture |
**this should allow us to do an SDS-PAGE on this first bioplastic gene | **this should allow us to do an SDS-PAGE on this first bioplastic gene | ||
**we're not sure if a lack of terminator on the gene will be problematic | **we're not sure if a lack of terminator on the gene will be problematic | ||
Revision as of 15:12, 17 July 2008
Overnight Results
- only plate with R0010 +A4 grew which is the same result as from the night before
- Which leads us to think our assemblies have some problem
- the assembly of BP2 and BP3 with the RBS(B0034) has failed twice in a row we need to look at the gel and make sure we are not making any false assumptions about the genes we have
Goals
- Grow E0240 in liquid broth from originally growing plate that came from the new DNA spot in the most recent newsletter
- we need to prove that this gene will glow green before we try to make it fluoresce in an assembly
- Use overnight broth of A6(R0010 + A4) from night before to miniprep and grow in a LAC liquid culture
- this should allow us to do an SDS-PAGE on this first bioplastic gene
- we're not sure if a lack of terminator on the gene will be problematic
- Digest and Assemble A6(R0010+A4) with a terminator(B0015) which we plan on also growing in a LAC liquid culture tomorrow
- Grow the measurement plasmid(psb1a10) on a plate with arabinose and in overnight liquid broth with and without arabinose
- plating with arabinose will prove that both genes in the vector will fluoresce
- overnight broth will allow us to prepare DNA by miniprepping so that we can insert terminators in between fluorescence
- Overnight broth with arabinose will be a double check to our work because we are not confident in our ability to make plates with arabinose
- Try assembling and plating BP2 and BP3 with the medium strength RBS instead of standard
- Start over with BP2 and BP3 grow a broth from the cells that were sent to us
