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Alberta NINT/14 July 2008
From 2008.igem.org
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Nothing grew from Friday's ligations so Wayne re-tranformed my ligations and carried out his own ligations | Nothing grew from Friday's ligations so Wayne re-tranformed my ligations and carried out his own ligations | ||
Plate # colonies | Plate # colonies | ||
| - | K102031W | + | K102031W 2 |
| - | K102021W | + | K102021W 1 |
| - | NegativeW | + | NegativeW 2 |
| - | K102031J | + | K102031J 0 |
| - | K102021J | + | K102021J 0 |
NegativeJ 0 | NegativeJ 0 | ||
Obviously, weekends ligations didn't work either. | Obviously, weekends ligations didn't work either. | ||
[[Alberta_NINT/15_July_2008 | Next entry >]] | [[Alberta_NINT/15_July_2008 | Next entry >]] | ||
Revision as of 17:47, 21 July 2008
lab work (SD):
Growth rates of cells containing the plasmids K102022, K102032, K102032+K102011, K102032+K102034,
K102032+K102035, K102032+K102036 and XL1-B were to be determined by measuring the cell count every
hour for cells grown at 30 and 37 C. Growth was only observed in overnights for K102022 and XL1-B.
t=1 hr t=2 hr t=3 hr t=4 hr
LB 0.000 0.000 0.000 0.000
K102022 (30 C) 0.128 0.162 0.192 0.247
K102022 (37 C) 0.167 0.278 0.351 0.407
XL1-B (30 C) 0.153 0.166 0.194 0.230
XL1-B (30 C) 0.147 0.238 0.344 0.345
lab work (JD):
Nothing grew from Friday's ligations so Wayne re-tranformed my ligations and carried out his own ligations
Plate # colonies
K102031W 2
K102021W 1
NegativeW 2
K102031J 0
K102021J 0
NegativeJ 0
Obviously, weekends ligations didn't work either.
