"
Alberta NINT/15 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: < Back to notebook < Previous entry lab work (SD): Since the double-plasmid cell cultures failed to grow in ...) |
|||
| Line 13: | Line 13: | ||
K102032+K102035 (LB+amp50+kan35) | K102032+K102035 (LB+amp50+kan35) | ||
K102032+K102036 (LB+amp50+kan35) | K102032+K102036 (LB+amp50+kan35) | ||
| + | |||
| + | lab work (JD): | ||
| + | We've decided to re-do the ligation another time from scratch: | ||
| + | Created O/N from glycerol stocks of: | ||
| + | pSB4A5 | ||
| + | K102003 | ||
| + | K102006 | ||
[[Alberta_NINT/16_July_2008 | Next entry >]] | [[Alberta_NINT/16_July_2008 | Next entry >]] | ||
Revision as of 17:50, 21 July 2008
lab work (SD):
Since the double-plasmid cell cultures failed to grow in LB+amp50+kan35 broth, perhaps the colonies
grown on LB+amp50+kan35 plates were not selected for kan resistance (ie. kan was not present on
these plates). New LB+amp50+kan35 plates were made and the transformation was redone.
K102022 (LB+amp50)
K102032 (LB+amp50)
K102032+K102011 (LB+amp50+kan35)
K102032+K102034 (LB+amp50+kan35)
K102032+K102035 (LB+amp50+kan35)
K102032+K102036 (LB+amp50+kan35)
lab work (JD):
We've decided to re-do the ligation another time from scratch:
Created O/N from glycerol stocks of:
pSB4A5
K102003
K102006
