Team:KULeuven/Primers

From 2008.igem.org

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| width=20% | Comments
| width=20% | Comments
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
-
|nr=1
+
|nr=1076
|name=T7 Fw, no tag
|name=T7 Fw, no tag
-
|sequence=catcatgaattcgcggccgcttctagatgaacacgattaacatcgc
+
|sequence=atgaattcgcggccgcttctagatgaacacgattaacatcgc
-
|length=46bp
+
|length=42bp
|comments=Forward T7 RNApol primer, without UmuD tag
|comments=Forward T7 RNApol primer, without UmuD tag
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
-
|nr=2
+
|nr=1077
|name=T7 Rev
|name=T7 Rev
|sequence=ctgcagcggccgctactagtattattacgcgaacgcgaagtccg
|sequence=ctgcagcggccgctactagtattattacgcgaacgcgaagtccg
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}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
-
|nr=3
+
|nr=1078
|name=T7 Fw, UmuD tag, part 1
|name=T7 Fw, UmuD tag, part 1
|sequence=ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc
|sequence=ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc
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}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
-
|nr=4
+
|nr=1079
|name=T7 Tw, UmuD tag, part 2
|name=T7 Tw, UmuD tag, part 2
-
|sequence=catcatgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag
+
|sequence=atgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag
-
|length=95bp
+
|length=91bp
|comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
|comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
-
|nr=5
+
|nr=1084
|name=GFP-LVA Fw
|name=GFP-LVA Fw
|sequence=catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg
|sequence=catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg
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}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
-
|nr=6
+
|nr=1085
|name=GFP-LVA Rev
|name=GFP-LVA Rev
|sequence=ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc
|sequence=ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc
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}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
-
|nr=7
+
|nr=1074
|name=VF2
|name=VF2
|sequence=TGCCACCTGACGTCTAAGAA
|sequence=TGCCACCTGACGTCTAAGAA
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}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
-
|nr=8
+
|nr=1075
|name=VR
|name=VR
|sequence=ATTACCGCCTTTGAGTGAGC
|sequence=ATTACCGCCTTTGAGTGAGC

Revision as of 13:51, 31 July 2008

  dock/undock dropdown  

Table of Oligos
Number Name Sequence Length Comments
1076 T7 Fw, no tag atgaattcgcggccgcttctagatgaacacgattaacatcgc 42bp {{{Tm}}} Forward T7 RNApol primer, without UmuD tag
1077 T7 Rev ctgcagcggccgctactagtattattacgcgaacgcgaagtccg 44bp {{{Tm}}} Reverse T7 RNApol primer
1078 T7 Fw, UmuD tag, part 1 ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc 62bp {{{Tm}}} Forward T7 RNApol primer, with UmuD tag, part 1
1079 T7 Tw, UmuD tag, part 2 atgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag 91bp {{{Tm}}} Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
1084 GFP-LVA Fw catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg 55bp {{{Tm}}} Forward GFP-LVA primer
1085 GFP-LVA Rev ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc 64bp {{{Tm}}} Reverse GFP-LVA primer
1074 VF2 TGCCACCTGACGTCTAAGAA 20bp {{{Tm}}} Forward primer for sequence analysis
1075 VR ATTACCGCCTTTGAGTGAGC 20bp {{{Tm}}} Reverse primer for sequence analysis