Team:KULeuven/Primers
From 2008.igem.org
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| width=20% | Comments | | width=20% | Comments | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
- | |nr= | + | |nr=1076 |
|name=T7 Fw, no tag | |name=T7 Fw, no tag | ||
- | |sequence= | + | |sequence=atgaattcgcggccgcttctagatgaacacgattaacatcgc |
- | |length= | + | |length=42bp |
|comments=Forward T7 RNApol primer, without UmuD tag | |comments=Forward T7 RNApol primer, without UmuD tag | ||
}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
- | |nr= | + | |nr=1077 |
|name=T7 Rev | |name=T7 Rev | ||
|sequence=ctgcagcggccgctactagtattattacgcgaacgcgaagtccg | |sequence=ctgcagcggccgctactagtattattacgcgaacgcgaagtccg | ||
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}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
- | |nr= | + | |nr=1078 |
|name=T7 Fw, UmuD tag, part 1 | |name=T7 Fw, UmuD tag, part 1 | ||
|sequence=ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc | |sequence=ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc | ||
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}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
- | |nr= | + | |nr=1079 |
|name=T7 Tw, UmuD tag, part 2 | |name=T7 Tw, UmuD tag, part 2 | ||
- | |sequence= | + | |sequence=atgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag |
- | |length= | + | |length=91bp |
|comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive | |comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive | ||
}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
- | |nr= | + | |nr=1084 |
|name=GFP-LVA Fw | |name=GFP-LVA Fw | ||
|sequence=catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg | |sequence=catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg | ||
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}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
- | |nr= | + | |nr=1085 |
|name=GFP-LVA Rev | |name=GFP-LVA Rev | ||
|sequence=ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc | |sequence=ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc | ||
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}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
- | |nr= | + | |nr=1074 |
|name=VF2 | |name=VF2 | ||
|sequence=TGCCACCTGACGTCTAAGAA | |sequence=TGCCACCTGACGTCTAAGAA | ||
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}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
- | |nr= | + | |nr=1075 |
|name=VR | |name=VR | ||
|sequence=ATTACCGCCTTTGAGTGAGC | |sequence=ATTACCGCCTTTGAGTGAGC |
Revision as of 13:51, 31 July 2008
dock/undock dropdown
Table of Oligos | |||||
---|---|---|---|---|---|
Number | Name | Sequence | Length | Comments | |
1076 | T7 Fw, no tag | atgaattcgcggccgcttctagatgaacacgattaacatcgc | 42bp | {{{Tm}}} | Forward T7 RNApol primer, without UmuD tag |
1077 | T7 Rev | ctgcagcggccgctactagtattattacgcgaacgcgaagtccg | 44bp | {{{Tm}}} | Reverse T7 RNApol primer |
1078 | T7 Fw, UmuD tag, part 1 | ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc | 62bp | {{{Tm}}} | Forward T7 RNApol primer, with UmuD tag, part 1 |
1079 | T7 Tw, UmuD tag, part 2 | atgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag | 91bp | {{{Tm}}} | Forward T7 RNApol primer, with UmuD tag, part 2, very expensive |
1084 | GFP-LVA Fw | catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg | 55bp | {{{Tm}}} | Forward GFP-LVA primer |
1085 | GFP-LVA Rev | ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc | 64bp | {{{Tm}}} | Reverse GFP-LVA primer |
1074 | VF2 | TGCCACCTGACGTCTAAGAA | 20bp | {{{Tm}}} | Forward primer for sequence analysis |
1075 | VR | ATTACCGCCTTTGAGTGAGC | 20bp | {{{Tm}}} | Reverse primer for sequence analysis |