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Team:KULeuven/Primers
From 2008.igem.org
(Difference between revisions)
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{{:Team:KULeuven/Tools/Header}} | {{:Team:KULeuven/Tools/Header}} | ||
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{|cellspacing="1" width=100% | {|cellspacing="1" width=100% | ||
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| width=5% | Number | | width=5% | Number | ||
| width=10% | Name | | width=10% | Name | ||
| - | | width= | + | | width=62% | Sequence |
| width=5% | Length | | width=5% | Length | ||
| width=20% | Comments | | width=20% | Comments | ||
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|nr=1076 | |nr=1076 | ||
|name=T7 Fw, no tag | |name=T7 Fw, no tag | ||
| - | |sequence= | + | |sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC</div> |
|length=42bp | |length=42bp | ||
|comments=Forward T7 RNApol primer, without UmuD tag | |comments=Forward T7 RNApol primer, without UmuD tag | ||
| Line 19: | Line 27: | ||
|nr=1077 | |nr=1077 | ||
|name=T7 Rev | |name=T7 Rev | ||
| - | |sequence= | + | |sequence= <div class="code">CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG</div> |
|length=44bp | |length=44bp | ||
|comments=Reverse T7 RNApol primer | |comments=Reverse T7 RNApol primer | ||
| Line 26: | Line 34: | ||
|nr=1078 | |nr=1078 | ||
|name=T7 Fw, UmuD tag, part 1 | |name=T7 Fw, UmuD tag, part 1 | ||
| - | |sequence= | + | |sequence= <div class="code">CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC</div> |
|length=62bp | |length=62bp | ||
|comments=Forward T7 RNApol primer, with UmuD tag, part 1 | |comments=Forward T7 RNApol primer, with UmuD tag, part 1 | ||
| Line 33: | Line 41: | ||
|nr=1079 | |nr=1079 | ||
|name=T7 Tw, UmuD tag, part 2 | |name=T7 Tw, UmuD tag, part 2 | ||
| - | |sequence= | + | |sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G</div> |
|length=91bp | |length=91bp | ||
|comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive | |comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive | ||
| Line 40: | Line 48: | ||
|nr=1084 | |nr=1084 | ||
|name=GFP-LVA Fw | |name=GFP-LVA Fw | ||
| - | |sequence= | + | |sequence= <div class="code">CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG</div> |
|length=55bp | |length=55bp | ||
|comments=Forward GFP-LVA primer | |comments=Forward GFP-LVA primer | ||
| Line 47: | Line 55: | ||
|nr=1085 | |nr=1085 | ||
|name=GFP-LVA Rev | |name=GFP-LVA Rev | ||
| - | |sequence= | + | |sequence= <div class="code">CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC |
|length=64bp | |length=64bp | ||
|comments=Reverse GFP-LVA primer | |comments=Reverse GFP-LVA primer | ||
| Line 54: | Line 62: | ||
|nr=1074 | |nr=1074 | ||
|name=VF2 | |name=VF2 | ||
| - | |sequence= | + | |sequence= <div class="code">TGCCACCTGA CGTCTAAGAA</div> |
|length=20bp | |length=20bp | ||
|comments=Forward primer for sequence analysis | |comments=Forward primer for sequence analysis | ||
| Line 61: | Line 69: | ||
|nr=1075 | |nr=1075 | ||
|name=VR | |name=VR | ||
| - | |sequence= | + | |sequence= <div class="code">ATTACCGCCT TTGAGTGAGC</div> |
|length=20bp | |length=20bp | ||
|comments=Reverse primer for sequence analysis | |comments=Reverse primer for sequence analysis | ||
}} | }} | ||
|} | |} | ||
Revision as of 14:18, 31 July 2008
dock/undock dropdown
| Table of Oligos | |||||
|---|---|---|---|---|---|
| Number | Name | Sequence | Length | Comments | |
| 1076 | T7 Fw, no tag | ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC
| 42bp | {{{Tm}}} | Forward T7 RNApol primer, without UmuD tag |
| 1077 | T7 Rev | CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG
| 44bp | {{{Tm}}} | Reverse T7 RNApol primer |
| 1078 | T7 Fw, UmuD tag, part 1 | CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC
| 62bp | {{{Tm}}} | Forward T7 RNApol primer, with UmuD tag, part 1 |
| 1079 | T7 Tw, UmuD tag, part 2 | ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G
| 91bp | {{{Tm}}} | Forward T7 RNApol primer, with UmuD tag, part 2, very expensive |
| 1084 | GFP-LVA Fw | CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG
| 55bp | {{{Tm}}} | Forward GFP-LVA primer |
| 1085 | GFP-LVA Rev | CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC
| 64bp | {{{Tm}}} | Reverse GFP-LVA primer |
| 1074 | VF2 | TGCCACCTGA CGTCTAAGAA
| 20bp | {{{Tm}}} | Forward primer for sequence analysis |
| 1075 | VR | ATTACCGCCT TTGAGTGAGC
| 20bp | {{{Tm}}} | Reverse primer for sequence analysis |
