"
Team:KULeuven/Project/CellDeath
From 2008.igem.org
(Difference between revisions)
(→BioBricks) |
|||
| Line 6: | Line 6: | ||
===BioBricks=== | ===BioBricks=== | ||
| - | + | [[Image:project_death.jpg|center]] | |
===Components=== | ===Components=== | ||
Revision as of 20:45, 31 July 2008
dock/undock dropdown
Contents |
Cell Death
BioBricks
Components
The luxR protein [http://partsregistry.org/Part:BBa_C0062 BBa_C0062] is placed under control of the constitutive promoter [http://partsregistry.org/Part:BBa_J23100 BBa_J23100]. The ccdB suicide gene [http://partsregistry.org/Part:BBa_P1010 BBa_P1010], on its part, is placed under control of the LuxpR promoter [http://partsregistry.org/Part:BBa_R0062 BBa_R0062].
Action
When the concentration HSL is big enough (see the modeling page), it will form a complex with the LuxR protein and thus activate the LuxpR promoter. This results in ccdB production and eventually cell death ([http://www.ncbi.nlm.nih.gov/pubmed/10196173?dopt=Abstract reference]).
