"
Team:UNIPV-Pavia/Notebook/Week12
From 2008.igem.org
(Difference between revisions)
(New page: <!--{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" --> {| style="color:#000000;background-color:#...) |
|||
| Line 57: | Line 57: | ||
*We had 5 plates to screen with colony PCR: | *We had 5 plates to screen with colony PCR: | ||
| - | ** | + | **BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-'''BBa_B1006'''-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062-BBa_B0030-BBa_E1010-BBa_B1006 |
| - | ** | + | **BBa_B0030-BBa_C0061-BBa_B1006-'''BBa_R0062'''-BBa_B0030-BBa_E0040-BBa_B1006 |
| + | **BBa_B0030-BBa_C0078-BBa_B1006-'''BBa_R0079'''-BBa_B0030-BBa_E0040-BBa_B1006 | ||
| + | **BBa_B0030-BBa_C0061-BBa_B0030-BBa_C0079-BBa_B1006-'''BBa_R0079'''-BBa_B0030-BBa_E0040-BBa_B1006 | ||
| + | **BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-'''BBa_R0040''' | ||
| + | |||
| + | *Last week BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-'''BBa_R0040''' colony PCR gave a bad result. For this reason, we decided to perform colony PCR noly for: | ||
| + | **BBa_B0030-BBa_C0061-BBa_B1006-'''BBa_R0062'''-BBa_B0030-BBa_E0040-BBa_B1006 (7 colonies) | ||
| + | **BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-'''BBa_R0040''' (6 colonies) | ||
{| | {| | ||
| - | |[[Image:pv_colonypcr_27_b.jpg|thumb|300px|left|]] | + | |[[Image:pv_colonypcr_27_b.jpg|thumb|300px|left|Marker 1Kb, blank, BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-'''BBa_R0040''' (6 colonies), BBa_B0030-BBa_C0061-BBa_B1006-'''BBa_R0062'''-BBa_B0030-BBa_E0040-BBa_B1006 (7 colonies)]] |
|} | |} | ||
| + | |||
| + | *Gel result: | ||
| + | **BBa_B0030-BBa_C0061-BBa_B1006-'''BBa_R0062'''-BBa_B0030-BBa_E0040-BBa_B1006 (2nd colony) | ||
| + | **BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-'''BBa_R0040''' (1st colony) | ||
Revision as of 20:46, 4 August 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|---|---|---|---|---|---|
| Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
Week 11: 04/28/08 - 08/8/08
08/4/08
- Plasmid digestion for:
| BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006 (E-S) | BBa_R0040 (E-X) |
- Gel run/cut/gel extraction.
- Ligation: BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040. We incubated ligation at 16°C overnight.
- We had 5 plates to screen with colony PCR:
- BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062-BBa_B0030-BBa_E1010-BBa_B1006
- BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062-BBa_B0030-BBa_E0040-BBa_B1006
- BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079-BBa_B0030-BBa_E0040-BBa_B1006
- BBa_B0030-BBa_C0061-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0079-BBa_B0030-BBa_E0040-BBa_B1006
- BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040
- Last week BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040 colony PCR gave a bad result. For this reason, we decided to perform colony PCR noly for:
- BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062-BBa_B0030-BBa_E0040-BBa_B1006 (7 colonies)
- BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040 (6 colonies)
 |
- Gel result:
- BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062-BBa_B0030-BBa_E0040-BBa_B1006 (2nd colony)
- BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040 (1st colony)
