Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
(→Parts stored in the fridge in the lab (4°C))
(→Things stored in freezer in lab (-20°C))
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! colspan="6" style="background: #003E81" | Miscellaneous
! colspan="6" style="background: #003E81" | Miscellaneous
|- style="background: #99CCFF; text-align: center; width=100%"
|- style="background: #99CCFF; text-align: center; width=100%"
-
|width=160| '''Description'''
+
|width=200| '''Description'''
|width=160| '''Name'''
|width=160| '''Name'''
-
|width=160| '''µl left'''
+
|width=150| '''µl left'''
-
|width=160| '''Concentration'''
+
|width=150| '''Concentration'''
-
|width=160| '''Box'''
+
|width=150| '''Box'''
-
|width=160| '''Date'''
+
|width=150| '''Date'''
|- style="background: #E0FFFF; text-align: center;"  
|- style="background: #E0FFFF; text-align: center;"  
-
|Primer||1084||990||20 µM||1||August 01
+
|Primer GFP-LVA||1084||90||20 µM||1||August 01
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|Primer||1085||990||20 µM||2||August 01
+
|Primer GFP-LVA||1085||90||20 µM||2||August 01
|- style="background: #E0FFFF; text-align: center;"
|- style="background: #E0FFFF; text-align: center;"
-
|dNTP mix||ATP, GTP, TTP, CTP||990||10 mM||3||August 01  
+
|dNTP mix||ATP, GTP, TTP, CTP||70||10 mM||3||August 01  
|- style="background: #E0FFFF; text-align: center;"
|- style="background: #E0FFFF; text-align: center;"
-
|Primer||1084||500||100 µM||4||August 01  
+
|Primer GFP-LVA||1084||500||100 µM||4||August 01  
|- style="background: #E0FFFF; text-align: center;"
|- style="background: #E0FFFF; text-align: center;"
-
|Primer||1085||500||100 µM||5||August 01  
+
|Primer GFP-LVA||1085||500||100 µM||5||August 01  
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Test||1074||800||100 µM||6||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Test||1075||780||100 µM||7||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer T7-UmuD||1076||560||100 µM||8||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer T7-UmuD||1077||477||100 µM||9||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer T7-UmuD||1078||467||100 µM||10||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer T7-UmuD||1079||45||100 µM||11||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Transduction||1097||627||100 µM||12||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Transduction||1098||710||100 µM||13||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Transduction||1099||608||100 µM||14||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Anti-sense LuxI||1100||522||100 µM||15||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Anti-sense LuxI||1101||237||100 µM||16||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Test||1074||100||20 µM||17||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Test||1075||100||20 µM||18||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer T7-UmuD||1076||100||20 µM||19||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer T7-UmuD||1077||100||20 µM||20||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer T7-UmuD||1078||100||20 µM||21||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer T7-UmuD||1079||100||20 µM||22||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Transduction||1097||100||20 µM||23||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Transduction||1098||100||20 µM||24||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Transduction||1099||100||20 µM||25||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Anti-sense LuxI||1100||100||20 µM||26||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer Anti-sense LuxI||1101||100||20 µM||27||August 07
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|dNTP mix||ATP, GTP, TTP, CTP||90||2 mM||28||August 07
|}
|}

Revision as of 14:03, 7 August 2008

  dock/undock dropdown  


Parts stored in the fridge in the lab (4°C)

Plasmids: MiniPrep Products
Part Plasmid Description µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 4050.41July 23
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 3054.32July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 60345.03 July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 30176.14 July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 2073.85July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 3095.16July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 40104.07July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 25177.18July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 30161.79July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 4063.010July 23
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 10112.311July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 20130.312July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 45303.113July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 30213.614July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 48311.031July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40214.047July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40209.548July 29
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 4032.015July 23
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 3060.916July 24
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS (efficiency 0.3)+/- 10114.818July 24
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4084.219July 23
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4086.420July 24
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI+/- 30133.121July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40146.449July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40203.150July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 35117.423July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 39.5144.824July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 48129.128July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 40160.725July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 46308.329July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 46375.126July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 4840430July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017 (Ap,Km)T7 Promotor+/- 42.5208.227July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 30104.022July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 35170.845July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 40159.046July 29
[http://partsregistry.org/Part:BBa_I712022 I712022]BBa_J52017 (Km, Ap)T7 polymerase+/- 40394.135July 31
[http://partsregistry.org/Part:BBa_R0062 R0062]pSB1A2LuxR regulated promoter +/- 40104.137July 31
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter+/- 4564.043July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter +/- 4069.844July 29
[http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 48119.138August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for TetR generator+/- 48131.039August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for TetR generator+/- 48133.240August 06
[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Reset+/- 48118.541August 06
[http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Cell Death+/- 4641.042August 06


Restriction Digests
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_J23100 J23100]EcoR ISpe IInsert5238.12.61July 24
[http://partsregistry.org/Part:BBa_C0040 C0040] EcoR ISpe IInsert677257.12July 24
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert12533.43.83July 24
[http://partsregistry.org/Part:BBa_E0022 E0022] EcoR ISpe IInsert779229.54July 24
[http://partsregistry.org/Part:BBa_J23109 J23109] EcoR ISpe IInsert52214.25July 29
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert125286.16July 29
[http://partsregistry.org/Part:BBa_I712074 I712074] EcoR ISpe IInsert62233.47July 29
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090212.98July 30
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090451.39July 31
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3308255.122August 07
[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR IXba IVector2077204.010July 31
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33033111.711July 31
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33032513.420August 07
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72208.512July 30
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72207.513July 30
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert7741824.714August 01
[http://partsregistry.org/Part:BBa_C0060 C0060] EcoR ISpe IInsert806158.215August 01
[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] EcoR ISpe IPlasmid20791718.216August 01
[http://partsregistry.org/Part:BBa_P1010 P1010] EcoR ISpe IInsert682104.917August 01
[http://partsregistry.org/Part:BBa_K145015 K145015] (GFP-LVA) EcoR ISpe IInsert625136.318August 05
[http://partsregistry.org/Part:BBa_C0012 C0012] EcoR ISpe IInsert1145255.619August 05
[http://partsregistry.org/Part:BBa_J23022 J23022] EcoR IXba IVector34982513.221August 07
[http://partsregistry.org/Part:BBa_R0062 R0062] EcoR ISpe IInsert55287.823August 07
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3305+/-255.122August 07

Things stored in freezer in lab (-20°C)

Miscellaneous
Description Name µl left Concentration Box Date
Primer GFP-LVA10849020 µM1August 01
Primer GFP-LVA10859020 µM2August 01
dNTP mixATP, GTP, TTP, CTP7010 mM3August 01
Primer GFP-LVA1084500100 µM4August 01
Primer GFP-LVA1085500100 µM5August 01
Primer Test1074800100 µM6August 07
Primer Test1075780100 µM7August 07
Primer T7-UmuD1076560100 µM8August 07
Primer T7-UmuD1077477100 µM9August 07
Primer T7-UmuD1078467100 µM10August 07
Primer T7-UmuD107945100 µM11August 07
Primer Transduction1097627100 µM12August 07
Primer Transduction1098710100 µM13August 07
Primer Transduction1099608100 µM14August 07
Primer Anti-sense LuxI1100522100 µM15August 07
Primer Anti-sense LuxI1101237100 µM16August 07
Primer Test107410020 µM17August 07
Primer Test107510020 µM18August 07
Primer T7-UmuD107610020 µM19August 07
Primer T7-UmuD107710020 µM20August 07
Primer T7-UmuD107810020 µM21August 07
Primer T7-UmuD107910020 µM22August 07
Primer Transduction109710020 µM23August 07
Primer Transduction109810020 µM24August 07
Primer Transduction109910020 µM25August 07
Primer Anti-sense LuxI110010020 µM26August 07
Primer Anti-sense LuxI110110020 µM27August 07
dNTP mixATP, GTP, TTP, CTP902 mM28August 07
Retrieved from "http://2008.igem.org/Team:KULeuven/Freezer"