Team:Hawaii/Notebook/2008-08- 9

From 2008.igem.org

(Difference between revisions)
(Plasmid prep)
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:* Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020
:* Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020
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::* Centrifuged for 75 min. at 4C after adding KoAc
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::* Centrifuged for 90 min. at 4C after adding KoAc
===Cryostock===
===Cryostock===

Revision as of 10:37, 10 August 2008

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Things we did today

Wetlab work

Plasmid prep

Grace
  • Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020
  • Centrifuged for 90 min. at 4C after adding KoAc

Cryostock

Grace
  • Did not take out 2.4 ml of E. coli before doing plasmid prep (oops). Reinoculated for cryostocking tomorrow.

Construction of p+r+g, g+t, J33207+t

Grace
  • RE digest of nir+rbs, plac+rbs, GFP, B0015, J33207
  • Digested nir+rbs, plac+rbs, J33207 with EcoRI and SpeI
  • Digested GFP, B0015 with Pst1 and XbaI
  • Ran digests on EtBr stained 2% agaorse gel
  • Gel did not run despite using new TAE buffer
  • Redid RE digest overnight


Gel Purification

Margaret
  • Ran the large-scale PCR of aadA(BB), aadA(pRL1383a), and P1 lytic region on a gel, cut it out, and purified it using the Qiagen Kit


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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