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Team:Valencia/Notebook/Protocols
From 2008.igem.org
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(→YPKAc Medium (Meri's medium)) |
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== Glycerol Stock == | == Glycerol Stock == | ||
2 ml Eppendorf containing: <br> | 2 ml Eppendorf containing: <br> | ||
| - | Sterile Glycerol (stock 50 %) | + | {| |
| - | Half culture plate <br> | + | |Sterile Glycerol (stock 50 %) |
| + | |1ml <br> | ||
| + | |- | ||
| + | |Half culture plate <br> | ||
| + | |} | ||
== SD Medium == | == SD Medium == | ||
| - | Yeast Nitrogen Base 0.67% | + | {| |
| - | Glucose | + | |Yeast Nitrogen Base |
| - | Agar | + | |0.67% |
| - | Casamino acids | + | |- |
| - | Tryptophan | + | |Glucose |
| - | Adenine | + | |2% |
| + | |- | ||
| + | |Agar | ||
| + | |2% | ||
| + | |- | ||
| + | |Casamino acids | ||
| + | |0.1% | ||
| + | |- | ||
| + | |Tryptophan | ||
| + | |20 mg/l | ||
| + | |- | ||
| + | |Adenine | ||
| + | |40 mg/l | ||
| + | |} | ||
Revision as of 12:39, 19 August 2008
Glycerol Stock
2 ml Eppendorf containing:
| Sterile Glycerol (stock 50 %) | 1ml |
| Half culture plate |
SD Medium
| Yeast Nitrogen Base | 0.67% |
| Glucose | 2% |
| Agar | 2% |
| Casamino acids | 0.1% |
| Tryptophan | 20 mg/l |
| Adenine | 40 mg/l |
SP liquid medium
Yeast Nitrogen Base 0.67%
Potassium phosohate 0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l
YPKAc Medium
Yeast extract 1%
Peptone 2%
Potassium acetate 2%
Tryptophan 20 mg/l
Adenine 40 mg/l
