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Edinburgh/27 June 2008
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Revision as of 11:04, 20 August 2008
Week 2
Friday 27 June 08
- Ordered Cellulomonas fimi ATCC 484 (NCIB 8980, DSM 20113) from DSMZ.
- Transformations of the iGEM competent JM109 cells with ligations L1 to L4. In each case, 100μl of cells were transformed with 5μl of ligation, and the remaining 5μl of each ligation was frozen so that it could be analysed if the transformations fail. Fresh Blue-White ampicillin plates were prepared.
Plate 3: Ligation L1, 100μl
Plate 4: Ligation L2, 100μl
Plate 5: Ligation L3, 100μl
Plate 6: Ligation L4, 100μl
Plate 7: Ligation L1, 900μl
Plate 8: Ligation L2, 900μl
Plate 9: Ligation L3, 900μl
Plate 10: Ligation L4, 900μl
