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Edinburgh/11 July 2008
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Revision as of 11:11, 20 August 2008
Week 4
Friday 11 July 08
- Submitted M19, M21 and M22 for sequencing using primer pTG262f1, to check for clean removal of the EcoRI site.
- Revived Cellulomonas fimi in NB and plated to NA and NA+glucose. Plates 28 and 29 incubated at 30°C.
- ZY and XH repeated rbs+dxs ligation (L11).
- Transformation of JM109 with L9 (pSB1A2+crtB )and L10 (pSB1A2+crtI-mut). Plates 30 to 33.
- possible rbs+dxs clones from un-numbered plates were streaked to plate 34.
