"
Edinburgh/15 July 2008
From 2008.igem.org
(Difference between revisions)
Andhi (Talk | contribs)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
Newer edit →
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
Newer edit →
Revision as of 11:14, 20 August 2008
Week 5
Tuesday 15 July 08
- PCR P15: fusion PCR of rbs+appY using L15 as template. Extension 20s, annealing 58°C, same primer set as before. Gel 13 shows that it worked OK.
- Gel 13 also shows digests of M31 to M42 from yesterday.
- Transformation of JM109 with ligations L13 and L15 (glgC mutation and rbs+dxs cloning). Plated to plates 39 to 41.
