"
Edinburgh/28 July 2008
From 2008.igem.org
(Difference between revisions)
Andhi (Talk | contribs)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
Newer edit →
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
Newer edit →
Revision as of 11:21, 20 August 2008
Week 7
Monday 28 July 08
- Repeated transformation of L20 (pSB1A2+rbs+appY) and L21 (pSB1A2+rbs+crtB), and plated on plates 63 to 66. (YAN and Omar).
- PCR of cenA, cenB, cenC and cex from cell suspension (P38-41; cell solution incubated at 73-80°C for 8 minutes to kill cells/inactivate DNAse) and DNA solution 'purified' from contaminated cell culture (P42-45). Denaturing 95°C for 1 min, annealing 55°C for 10s, extension 70°C for 90s. PCR products run on Gel 26. (AM)
- Prepared L25 of P36 (glgC-mut1,2 PCRed with primers f2 and r2) and Edinbrick 1. (AM)
- Minipreps M49 to M54 of pSB1A2+rbs+crtI transformants. (CF)
