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Edinburgh/4 August 2008
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Revision as of 11:30, 20 August 2008
Week 8
Monday 4 August 08
- Made 17 minipreps from transformations of L26, L27, L28, L29: M80-83 (L26: rbs+appY in Edinbrick1), M84-85 (L27: rbs+crtB in Edinbrick1), M86-90 (L28: cenA in Edinbrick1) and M91-96 (L29: cex in Edinbrick1). This was followed by double digestion of all the miniprep products (M80-96) with EcoRI/PstI. (Yan, OG)
- Third glgC mutagenesis made by MABEL on BABEL2+glgC-mut1,2 (since pSB1A2+glgC-mut1,2 appears to have failed) performed: P50 (Yan)
- Double digestions of M80 to M96 and P50 were run on gel 36. Results were good for M80-82 (pSB1A2+rbs+appY), M84-85 (pSB1A2+rbs+crtB), M92 (pSB1A2+cex) and P50 (glgC-mut1,2,3). Other results were poor, with bands in the wrong position for M86-M90 (pSB1A2+cenA). (Yan, OG)
- Sequencing results back today indicate that M50 = pSB1A2+rbs+crtI, M63 = pSB1A2+rbs+crtE and M67 = pSB1A2+rbs+crtB. (AH)
