Edinburgh/8 August 2008

From 2008.igem.org

(Difference between revisions)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
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</html>
</html>
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=== Week 8 ===
 
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<title>Edinburgh iGem 2008</title>
 
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<script type="text/javascript">
 
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
 
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//Created: May 16th, 07'
 
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this.showhide(this.dropmenuobj.style, e, obj)
 
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this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
 
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this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
 
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this.positionshim() //call iframe shim function
 
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while (b.parentNode)
 
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if ((b = b.parentNode) == a)
 
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var evtobj=window.event? window.event : e
 
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if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
 
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this.delayhidemenu(obj2)
 
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menuitems[i].parentNode.className+=" selected default"
 
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<style>
 
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padding: 0;
 
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voice-family: "\"}\"";
 
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/* ######### Style for Drop Down Menu ######### */
 
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.dropmenudiv_a{
 
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position:absolute;
 
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top: 0;
 
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border: 1px solid black; /*THEME CHANGE HERE*/
 
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border-bottom-width: 0;
 
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font:normal 12px Arial;
 
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line-height:18px;
 
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z-index:100;
 
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.dropmenudiv_a a{
 
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border-top: 0 solid #678b3f;
 
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border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
 
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padding: 2px 0;
 
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text-decoration: none;
 
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color: black;
 
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}
 
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* html .dropmenudiv_a a{ /*IE only hack*/
 
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width: 100%;
 
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}
 
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
 
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background-color: #8a3c3d;
 
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color: white;
 
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}
 
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</style>
 
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</head>
 
-
 
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<!-- CSS for Drop Down Tabs Menu #1 -->
 
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
 
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<div id="colortab" class="ddcolortabs">
 
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<ul>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
 
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
 
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</ul>
 
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</div>
 
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<div class="ddcolortabsline">&nbsp;</div>
 
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<div id="dropmenu1_a" class="dropmenudiv_a">
 
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
 
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
 
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
 
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</div>
 
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<script type="text/javascript">
 
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//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
 
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tabdropdown.init("colortab", 3)
 
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</script>
 
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</html>
 
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For more information, please see [https://www.wiki.ed.ac.uk/display/iGEM2008/HOME our internal wiki].
 
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= Calender =
 
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<table>
 
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<html>
 
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<style type="text/css">
 
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table.calendar          { margin: 0; padding: 2px; }
 
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table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; }
 
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table.month .dow td    { color:#000000; text-align:center; font-size:100%; }
 
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table.month td.today    { background-color:#cd0000; }
 
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table.month td {
 
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    border: none;
 
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    margin: 0;
 
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    padding: 0pt 0.5pt;
 
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    font-weight: bold;
 
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    font-size: 9pt;
 
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    text-align: right;
 
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    background-color: white;
 
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#bodyContent table.month a { background:none; padding:0 }
 
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.day-active { color:#cd0000 }
 
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.day-empty  { color:#000000 }
 
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</style>
 
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</html>
 
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{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:center" width="250px"
 
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<h3>Calendar</h3>
 
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|-
 
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|
 
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<td><td> {{#calendar: title=Edinburgh |year=2008 | month=06}}
 
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<td><td> {{#calendar: title=Edinburgh |year=2008 | month=07}}
 
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<td><td> {{#calendar: title=Edinburgh |year=2008 | month=08}}
 
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</td></tr>
 
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|-
 
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|
 
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</table>
 
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= Lab Notebook =
 
=== Week 8 ===
=== Week 8 ===
==== Friday 8 August 08 ====
==== Friday 8 August 08 ====

Revision as of 11:32, 20 August 2008

Edinburgh iGEM 2008

 

Week 8

Friday 8 August 08

  • Plate 94 (BABEL2+glgC-mut1,2,3) has white cultures, so can be prepped for minipreps. Plate 95 (pSB1A2+glgC-mut1,2) has blue cultures. Therefore transformation of L31 (glgC-mut1,2 to Edinbrick1) has been repeated on plates 100 and 101. (HX)
  • Transformation of L32 (pSB1A2+crtB+crtI) on plates 98 and 99. (HX)
  • Digested pCstA (P54) and Edinbrick1 using Spe1/EcoR1. Ligated as L33 to be transformed tomorrow (OG)
  • Arabidopsis isa1 and isa2 cDNAs have arrived as well as Zea mays su1, iso2 and iso3. The Arabidopsis genes are full of forbidden restriction sites, so it would be best to use the maize genes. Primers will need to be designed. (AH)
  • PCRed cenA and cex with a) KOD polymerase, b) Velocity polymerase separately to make P55 (cenA by KOD), P56 (cenA by Velocity), P57 (cex by KOD) and P58 (cex by Velocity). (Yan)
  • Combined rbs+dxs with rbs+crtE as L34, and rbs+dxs with rbs+lims1 as L35. L34 and L35 ligation is being allowed to occur at 16°C overnight. (Yan)
  • M79 sequencing results indicate that M79 is BABEL2+crtE. (AH)