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Edinburgh/9 July 2008
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| - | * Results of L6 and L7 transformations: no colonies for L6, but 9 colonies were obtained from L7. These were subcultured to '''plates 26 and 27'''. By the end of the day, these had grown sufficiently for 6 overnight cultures to be set up for minipreps tomorrow. In theory, some or all of these should be ''glgC'' with EcoRI site 2 mutated out. | + | </style> |
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
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| + | :::: '''[[Edinburgh/8_July_2008|< Previous Entry]]''' | ||
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| + | == Week 4 == | ||
| + | === Wednesday 9 July 08 === | ||
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| + | * Results of L6 (BABEL2+''glgC''-mut1) and L7 (BABEL2+''glgC''-mut2) transformations: no colonies for L6, but 9 colonies were obtained from L7. These were subcultured to '''plates 26 and 27'''. By the end of the day, these had grown sufficiently for 6 overnight cultures to be set up for minipreps tomorrow. In theory, some or all of these should be ''glgC'' with EcoRI site 2 mutated out. | ||
* Minipreps '''M13 to M18''' of possible pSB1A2+''appY' transformants. Digests with EcoRI/PstI were run on '''Gel 10'''. All but M13 looked OK (insert band on M14 was so faint as to be ambiguous, but M15 to M18 all look good). | * Minipreps '''M13 to M18''' of possible pSB1A2+''appY' transformants. Digests with EcoRI/PstI were run on '''Gel 10'''. All but M13 looked OK (insert band on M14 was so faint as to be ambiguous, but M15 to M18 all look good). | ||
| - | * Digests and ligation '''L8''' for addition of a synthetic ribosome binding site M2 (which has now been entered into the Registry as '''BBa_K118000'''). | + | * Digests and ligation '''L8''' for addition of a synthetic ribosome binding site M2 (which has now been entered into the Registry as '''BBa_K118000''').<br /> |
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| + | :::: '''[[Edinburgh/10_July_2008|Next Entry >]]''' | ||
Revision as of 11:55, 21 August 2008
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Week 4
Wednesday 9 July 08
- Results of L6 (BABEL2+glgC-mut1) and L7 (BABEL2+glgC-mut2) transformations: no colonies for L6, but 9 colonies were obtained from L7. These were subcultured to plates 26 and 27. By the end of the day, these had grown sufficiently for 6 overnight cultures to be set up for minipreps tomorrow. In theory, some or all of these should be glgC with EcoRI site 2 mutated out.
- Minipreps M13 to M18 of possible pSB1A2+appY' transformants. Digests with EcoRI/PstI were run on Gel 10. All but M13 looked OK (insert band on M14 was so faint as to be ambiguous, but M15 to M18 all look good).
- Digests and ligation L8 for addition of a synthetic ribosome binding site M2 (which has now been entered into the Registry as BBa_K118000).
