Edinburgh/14 July 2008
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* Digests and ligation '''L12''' to add rbs to pSB1A2+''appY'' clone M15 (sequence back today, seems OK). | * Digests and ligation '''L12''' to add rbs to pSB1A2+''appY'' clone M15 (sequence back today, seems OK). | ||
* Minipreps '''M31 to M36''' of ''crtB'' clones from plate 35 and '''M37 to to M42''' of ''crtI'' clones from plate 36. Digested with EcoRI/PstI. (AM) | * Minipreps '''M31 to M36''' of ''crtB'' clones from plate 35 and '''M37 to to M42''' of ''crtI'' clones from plate 36. Digested with EcoRI/PstI. (AM) | ||
- | * PCR '''P13''' and '''P14''' to | + | * PCR '''P13''' and '''P14''' to mutate out first EcoRI site from BABEL2+''glgC''-mut2 clones M19 and M22. These were ran on '''Gel 13'''. |
+ | * Self-ligations of P13 and P14 ('''L13'' and '''L14) set up.<br /> | ||
<br /> | <br /> | ||
:::: '''[[Edinburgh/15_July_2008|Next Entry >]]''' | :::: '''[[Edinburgh/15_July_2008|Next Entry >]]''' |
Revision as of 15:35, 21 August 2008
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Week 5
Monday 14 July 08
- Digests and ligation L12 to add rbs to pSB1A2+appY clone M15 (sequence back today, seems OK).
- Minipreps M31 to M36 of crtB clones from plate 35 and M37 to to M42 of crtI clones from plate 36. Digested with EcoRI/PstI. (AM)
- PCR P13 and P14 to mutate out first EcoRI site from BABEL2+glgC-mut2 clones M19 and M22. These were ran on Gel 13.
- Self-ligations of P13 and P14 (L13 and L14) set up.