Team:Hawaii/Notebook/2008-08-20

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Revision as of 02:45, 23 August 2008

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Things we did today

Wetlab work

Name of Task

Annealing was quite successful in that there is only one BRIGHT band.
Margaret & Krystle
  • Annealed and phosphorylated:
  • plac (I14032) for front ligation
  • rbs 1.0 (B0034) for back ligation
  • rbs 0.6 (B0030) for front ligation
  • tt (B1006) for back ligation
  • We decided to perform this reaction again with out gel purification and the gel was discarded.
  • These annealed products can be found in the -20°C freezer in the DNA box.
  • Quantification was achieved simply by adding up the amount of DNA added.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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