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Team:Hawaii/Notebook/2008-08-20
From 2008.igem.org
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::* plac (I14032) for front ligation | ::* plac (I14032) for front ligation | ||
::* rbs 1.0 (B0034) for back ligation | ::* rbs 1.0 (B0034) for back ligation | ||
| + | ::* rbs 0.6 (B0030) for back ligation | ||
::* rbs 0.6 (B0030) for front ligation | ::* rbs 0.6 (B0030) for front ligation | ||
::* tt (B1006) for back ligation | ::* tt (B1006) for back ligation | ||
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::* rbs 1.0 (B0034) for back ligation --> 206.7ng/ul | ::* rbs 1.0 (B0034) for back ligation --> 206.7ng/ul | ||
::* rbs 0.6 (B0030) for front ligation --> | ::* rbs 0.6 (B0030) for front ligation --> | ||
| + | ::* rbs 0.6 (B0030) for back ligation --> 228.3ng/ul | ||
::* tt (B1006) for back ligation --> 375ng/ul | ::* tt (B1006) for back ligation --> 375ng/ul | ||
Revision as of 03:10, 23 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Name of Task
- Margaret & Krystle
- Annealed and phosphorylated:
- plac (I14032) for front ligation
- rbs 1.0 (B0034) for back ligation
- rbs 0.6 (B0030) for back ligation
- rbs 0.6 (B0030) for front ligation
- tt (B1006) for back ligation
- We decided to perform this reaction again with out gel purification and the gel was discarded.
- These annealed products can be found in the -20°C freezer in the DNA box.
- Quantification was achieved simply by adding up the amount of DNA added.
- plac (I14032) for front ligation --> 370ng/ul
- rbs 1.0 (B0034) for back ligation --> 206.7ng/ul
- rbs 0.6 (B0030) for front ligation -->
- rbs 0.6 (B0030) for back ligation --> 228.3ng/ul
- tt (B1006) for back ligation --> 375ng/ul
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
