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Team:KULeuven/26 August 2008
From 2008.igem.org
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* Looks like we've finally nailed the transduction-PCR. The photo of our gel looks very promissing, tomorrow we'll discuss it with our advisors, but we think our cells should be fine! | * Looks like we've finally nailed the transduction-PCR. The photo of our gel looks very promissing, tomorrow we'll discuss it with our advisors, but we think our cells should be fine! | ||
* We cut B0015 and F1610+R0011 with ''Xba''I and ''Eco''RI. There is something wrong with F1610+R0011, so we have to do this again. | * We cut B0015 and F1610+R0011 with ''Xba''I and ''Eco''RI. There is something wrong with F1610+R0011, so we have to do this again. | ||
| - | * The following parts were | + | * The following parts were electroporated: K145001+pSB1A2 and K145151+pSB1A2. |
* We made liquid cultures of some BioBricks. | * We made liquid cultures of some BioBricks. | ||
* We did a PCR to test the ligations. We are not sure of the ligations J23116+B0032 and ''J23022+R0040''. The other ones seem to be OK. | * We did a PCR to test the ligations. We are not sure of the ligations J23116+B0032 and ''J23022+R0040''. The other ones seem to be OK. | ||
Revision as of 19:10, 26 August 2008
dock/undock dropdown
Contents |
Lab Work
Wet Lab
- Looks like we've finally nailed the transduction-PCR. The photo of our gel looks very promissing, tomorrow we'll discuss it with our advisors, but we think our cells should be fine!
- We cut B0015 and F1610+R0011 with XbaI and EcoRI. There is something wrong with F1610+R0011, so we have to do this again.
- The following parts were electroporated: K145001+pSB1A2 and K145151+pSB1A2.
- We made liquid cultures of some BioBricks.
- We did a PCR to test the ligations. We are not sure of the ligations J23116+B0032 and J23022+R0040. The other ones seem to be OK.
Dry Lab
Alright, here we go. The two parallel schemes for building the system:
General meeting with the team decided upon some important/emergency building-stuff :)
We've started on thinking about the ethical part of our project. We would start from the film "I, Robot" and then focus further on the laws of robotics. Benjamien and Hanne did a lot of research on probiotics, ethics and genetic engineering.
Modeling
Some more stochastic stuff
Wiki
General modeling page updates.
Remarks
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