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Edinburgh/7 August 2008
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | ||
<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | ||
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | ||
| - | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> | + | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> |
| + | <li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li> | ||
| + | <li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li> | ||
| + | <li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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| - | === | + | <table> |
| - | ==== Thursday 7 August 08 | + | <html> |
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| + | </table> | ||
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| + | {| align="left" | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
| + | |} | ||
| + | |||
| + | :::: '''[[Edinburgh/6_August_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 8 == | ||
| + | === Thursday 7 August 08 === | ||
* Subcultured from Plates 89 and 90 (BABEL2+''glgC''-mut1,2,3) to '''Plate 94'''. Plate 91 (pSB1A2+''glgC''-mut1,2) showed no growth, so was discarded. Plate 92 (pSB1A2+''glgC''-mut1,2) had a blue smear and two white colonies. These colonies were subbed to '''Plate 95'''. (HX) | * Subcultured from Plates 89 and 90 (BABEL2+''glgC''-mut1,2,3) to '''Plate 94'''. Plate 91 (pSB1A2+''glgC''-mut1,2) showed no growth, so was discarded. Plate 92 (pSB1A2+''glgC''-mut1,2) had a blue smear and two white colonies. These colonies were subbed to '''Plate 95'''. (HX) | ||
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* PCR '''P54''' of pCstA. Run on '''gel 40'''. Results look promising (fairly prominent band <500bp). Purified. (CF) | * PCR '''P54''' of pCstA. Run on '''gel 40'''. Results look promising (fairly prominent band <500bp). Purified. (CF) | ||
* Plates made for testing glycogen assay. 2x LB agar, ampicillin; 2x LB agar, ampicillin, 2% glucose ready for spreading with ''E. coli''. (SK) | * Plates made for testing glycogen assay. 2x LB agar, ampicillin; 2x LB agar, ampicillin, 2% glucose ready for spreading with ''E. coli''. (SK) | ||
| - | * '''Plates 96 and 97''' (glycogen assay) were spread with subcultures from Plate 43. Plate 96 = (-)glucose, plate 97 = (+)glucose. (HX) | + | * '''Plates 96 and 97''' (glycogen assay) were spread with subcultures from Plate 43. Plate 96 = (-)glucose, plate 97 = (+)glucose. (HX)<br /> |
| + | <br /> | ||
| + | |||
| + | :::: '''[[Edinburgh/8_August_2008|Next Entry >]]''' | ||
Revision as of 10:20, 28 August 2008
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Week 8
Thursday 7 August 08
- Subcultured from Plates 89 and 90 (BABEL2+glgC-mut1,2,3) to Plate 94. Plate 91 (pSB1A2+glgC-mut1,2) showed no growth, so was discarded. Plate 92 (pSB1A2+glgC-mut1,2) had a blue smear and two white colonies. These colonies were subbed to Plate 95. (HX)
- Maxiprep X8 (as M67: pSB1A2+rbs+crtB) made. Slight hiccup after step 6. (Cold solution 3 was added and incubation on ice was skipped. Centrifugation continued for 2 minutes before mistake was realised, after which the solution was mixed and incubated on ice for ten minutes (continuing with step 7). (AM, AH)
- Combination (Ligation) of rbs+crtB (from M67) and rbs+crtI (from M50): rbs+crtB as an insert cut with EcoRI/SpeI; rbs+crtI as a vector cut with EcoRI/XbaI (L32). (Yan, AM)
- Double digestion of three biobricks: 2 of rbs+dxs (from M72), rbs+crtE (from M63) and rbs+lims1 (from 0713536 in iGEM 07 box): rbs+dxs as a vector cut with SpeI/PstI; rbs+crtE as an insert cut with XbaI/PstI; rbs+lims1 as an insert cut with XbaI/PstI.
- Made PCR of cenA and cex again, stored in P52 (cenA) and P53 (cex). The PCR products were checked on gel, but failed again! (Yan, AM)
- PCR P54 of pCstA. Run on gel 40. Results look promising (fairly prominent band <500bp). Purified. (CF)
- Plates made for testing glycogen assay. 2x LB agar, ampicillin; 2x LB agar, ampicillin, 2% glucose ready for spreading with E. coli. (SK)
- Plates 96 and 97 (glycogen assay) were spread with subcultures from Plate 43. Plate 96 = (-)glucose, plate 97 = (+)glucose. (HX)
