Edinburgh/8 August 2008

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(→Friday 8 August 08)
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=== Friday 8 August 08 ===
=== Friday 8 August 08 ===
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* Plate 94 (BABEL2+''glgC''-mut1,2,3) has white cultures, so can be prepped for minipreps. Plate 95 (pSB1A2+''glgC''-mut1,2) has blue cultures. Therefore transformation of L31 (''glgC''-mut1,2 to Edinbrick1) has been repeated on '''plates 100 and 101'''. (HX)
+
* Transformation of L32 (pSB1A2+''crtB''+''crtI'') on '''plates 98-99'''. (HX)
-
* Transformation of L32 (pSB1A2+''crtB''+''crtI'') on '''plates 98 and 99'''. (HX)
+
* Plate 94 (BABEL2+''glgC''-mut1,2,3) has white colonies, so can be prepped for minipreps. Plate 95 (pSB1A2+''glgC''-mut1,2) has blue colonies. Because of this, transformation of L31 (''glgC''-mut1,2 to Edinbrick1) has been repeated on '''plates 100-101'''. (HX)
-
* Digested pCstA (P54) and Edinbrick1 using Spe1/EcoR1. Ligated as '''L33''' to be transformed tomorrow (OG)
+
* Digested pCstA (P54) and Edinbrick1 using Spe1/EcoR1. Ligated as '''L33''', to be transformed tomorrow (OG)
-
* ''Arabidopsis'' ''isa1'' and ''isa2'' cDNAs have arrived as well as ''Zea mays'' ''su1'', ''iso2'' and ''iso3''. The ''Arabidopsis'' genes are full of forbidden restriction sites, so it would be best to use the maize genes. Primers will need to be designed. (AH)
+
* ''Arabidopsis thaliana'' ''isa1'' and ''isa2'' cDNAs have arrived as well as ''Zea mays'' ''su1'', ''iso2'' and ''iso3''. The ''Arabidopsis'' genes are full of forbidden restriction sites, so it would be best to use the maize genes. Primers will need to be designed. (AH)
* PCRed ''cenA'' and ''cex'' with a) KOD polymerase, b) Velocity polymerase separately to make '''P55''' (''cenA'' by KOD), '''P56''' (''cenA'' by Velocity), '''P57''' (''cex'' by KOD) and '''P58''' (''cex'' by Velocity). (Yan)
* PCRed ''cenA'' and ''cex'' with a) KOD polymerase, b) Velocity polymerase separately to make '''P55''' (''cenA'' by KOD), '''P56''' (''cenA'' by Velocity), '''P57''' (''cex'' by KOD) and '''P58''' (''cex'' by Velocity). (Yan)
-
* Combined rbs+''dxs'' with rbs+''crtE'' as '''L34''', and rbs+''dxs'' with rbs+''lims1'' as '''L35'''. L34 and L35 ligation is being allowed to occur at 16°C overnight. (Yan)
+
* Ligated rbs+''dxs'' with rbs+''crtE'' as '''L34''' (pSB1A2+''dxs''+''crtE'') and rbs+''dxs'' with rbs+''lims1'' as '''L35''' (pSB1A2+''dxs''+''lims1''). (Yan)
* M79 sequencing results indicate that M79 is BABEL2+''crtE''. (AH)<br />
* M79 sequencing results indicate that M79 is BABEL2+''crtE''. (AH)<br />
<br />
<br />
:::: '''[[Edinburgh/10_August_2008|Next Entry >]]'''
:::: '''[[Edinburgh/10_August_2008|Next Entry >]]'''

Revision as of 11:45, 28 August 2008

Edinburgh iGEM 2008

 

June
MTWTFSS
            [http://2008.igem.org/wiki/index.php?title=Edinburgh/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_June_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Edinburgh/3_June_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Edinburgh/4_June_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Edinburgh/5_June_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Edinburgh/6_June_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Edinburgh/7_June_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Edinburgh/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/9_June_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Edinburgh/10_June_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Edinburgh/11_June_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Edinburgh/12_June_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Edinburgh/13_June_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_June_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Edinburgh/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/16_June_2008&action=edit 16] [http://2008.igem.org/Edinburgh/17_June_2008 17] [http://2008.igem.org/wiki/index.php?title=Edinburgh/18_June_2008&action=edit 18] [http://2008.igem.org/Edinburgh/19_June_2008 19] [http://2008.igem.org/Edinburgh/20_June_2008 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_June_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Edinburgh/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_June_2008&action=edit 23] [http://2008.igem.org/Edinburgh/24_June_2008 24] [http://2008.igem.org/Edinburgh/25_June_2008 25] [http://2008.igem.org/Edinburgh/26_June_2008 26] [http://2008.igem.org/Edinburgh/27_June_2008 27] [http://2008.igem.org/Edinburgh/28_June_2008 28] [http://2008.igem.org/Edinburgh/29_June_2008 29]
[http://2008.igem.org/Edinburgh/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Edinburgh/1_July_2008 1] [http://2008.igem.org/Edinburgh/2_July_2008 2] [http://2008.igem.org/Edinburgh/3_July_2008 3] [http://2008.igem.org/Edinburgh/4_July_2008 4] [http://2008.igem.org/Edinburgh/5_July_2008 5] [http://2008.igem.org/Edinburgh/6_July_2008 6]
[http://2008.igem.org/Edinburgh/7_July_2008 7] [http://2008.igem.org/Edinburgh/8_July_2008 8] [http://2008.igem.org/Edinburgh/9_July_2008 9] [http://2008.igem.org/Edinburgh/10_July_2008 10] [http://2008.igem.org/Edinburgh/11_July_2008 11] [http://2008.igem.org/Edinburgh/12_July_2008 12] [http://2008.igem.org/Edinburgh/13_July_2008 13]
[http://2008.igem.org/Edinburgh/14_July_2008 14] [http://2008.igem.org/Edinburgh/15_July_2008 15] [http://2008.igem.org/Edinburgh/16_July_2008 16] [http://2008.igem.org/Edinburgh/17_July_2008 17] [http://2008.igem.org/Edinburgh/18_July_2008 18] [http://2008.igem.org/Edinburgh/19_July_2008 19] [http://2008.igem.org/Edinburgh/20_July_2008 20]
[http://2008.igem.org/Edinburgh/21_July_2008 21] [http://2008.igem.org/Edinburgh/22_July_2008 22] [http://2008.igem.org/Edinburgh/23_July_2008 23] [http://2008.igem.org/Edinburgh/24_July_2008 24] [http://2008.igem.org/Edinburgh/25_July_2008 25] [http://2008.igem.org/Edinburgh/26_July_2008 26] [http://2008.igem.org/Edinburgh/27_July_2008 27]
[http://2008.igem.org/Edinburgh/28_July_2008 28] [http://2008.igem.org/Edinburgh/29_July_2008 29] [http://2008.igem.org/Edinburgh/30_July_2008 30] [http://2008.igem.org/Edinburgh/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Edinburgh/1_August_2008 1] [http://2008.igem.org/wiki/index.php?title=Edinburgh/2_August_2008&action=edit 2] [http://2008.igem.org/Edinburgh/3_August_2008 3]
[http://2008.igem.org/Edinburgh/4_August_2008 4] [http://2008.igem.org/Edinburgh/5_August_2008 5] [http://2008.igem.org/Edinburgh/6_August_2008 6] [http://2008.igem.org/Edinburgh/7_August_2008 7] [http://2008.igem.org/Edinburgh/8_August_2008 8] [http://2008.igem.org/Edinburgh/9_August_2008 9] [http://2008.igem.org/Edinburgh/10_August_2008 10]
[http://2008.igem.org/Edinburgh/11_August_2008 11] [http://2008.igem.org/Edinburgh/12_August_2008 12] [http://2008.igem.org/Edinburgh/13_August_2008 13] [http://2008.igem.org/Edinburgh/14_August_2008 14] [http://2008.igem.org/Edinburgh/15_August_2008 15] [http://2008.igem.org/Edinburgh/16_August_2008 16] [http://2008.igem.org/Edinburgh/17_August_2008 17]
[http://2008.igem.org/Edinburgh/18_August_2008 18] [http://2008.igem.org/Edinburgh/19_August_2008 19] [http://2008.igem.org/Edinburgh/20_August_2008 20] [http://2008.igem.org/Edinburgh/21_August_2008 21] [http://2008.igem.org/Edinburgh/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Edinburgh/23_August_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Edinburgh/24_August_2008&action=edit 24]
[http://2008.igem.org/Edinburgh/25_August_2008 25] [http://2008.igem.org/Edinburgh/26_August_2008 26] [http://2008.igem.org/Edinburgh/27_August_2008 27] [http://2008.igem.org/Edinburgh/28_August_2008 28] [http://2008.igem.org/Edinburgh/29_August_2008 29] [http://2008.igem.org/wiki/index.php?title=Edinburgh/30_August_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Edinburgh/31_August_2008&action=edit 31]
< Previous Entry

Week 8

Friday 8 August 08

  • Transformation of L32 (pSB1A2+crtB+crtI) on plates 98-99. (HX)
  • Plate 94 (BABEL2+glgC-mut1,2,3) has white colonies, so can be prepped for minipreps. Plate 95 (pSB1A2+glgC-mut1,2) has blue colonies. Because of this, transformation of L31 (glgC-mut1,2 to Edinbrick1) has been repeated on plates 100-101. (HX)
  • Digested pCstA (P54) and Edinbrick1 using Spe1/EcoR1. Ligated as L33, to be transformed tomorrow (OG)
  • Arabidopsis thaliana isa1 and isa2 cDNAs have arrived as well as Zea mays su1, iso2 and iso3. The Arabidopsis genes are full of forbidden restriction sites, so it would be best to use the maize genes. Primers will need to be designed. (AH)
  • PCRed cenA and cex with a) KOD polymerase, b) Velocity polymerase separately to make P55 (cenA by KOD), P56 (cenA by Velocity), P57 (cex by KOD) and P58 (cex by Velocity). (Yan)
  • Ligated rbs+dxs with rbs+crtE as L34 (pSB1A2+dxs+crtE) and rbs+dxs with rbs+lims1 as L35 (pSB1A2+dxs+lims1). (Yan)
  • M79 sequencing results indicate that M79 is BABEL2+crtE. (AH)


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