Edinburgh/10 August 2008
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* Extracted ''C. fimi'' genomic DNA, followed by PCR of ''rrnB'' ('''P61'''), ''cenA'' ('''P62''') and ''cex'' ('''P63''') from genomic DNA. (CF) | * Extracted ''C. fimi'' genomic DNA, followed by PCR of ''rrnB'' ('''P61'''), ''cenA'' ('''P62''') and ''cex'' ('''P63''') from genomic DNA. (CF) | ||
* Transformed competent cells with L36, pSB1A2+''crtY'' ('''Plates 104-105''') and L37, pSB1A2+rbs+''crtY'' ('''Plates 106-107'''). (CF) | * Transformed competent cells with L36, pSB1A2+''crtY'' ('''Plates 104-105''') and L37, pSB1A2+rbs+''crtY'' ('''Plates 106-107'''). (CF) | ||
- | * Set-up cultures from plates 102 (pSB1A2+''crtB''+''crtI'') and 103 (pSB1A2+''glgC''-mut1,2) for miniprep. (CF)<br /> | + | * Set-up cultures from plates 102 (pSB1A2+''crtB''+''crtI'') and 103 (pSB1A2+''glgC''-mut1,2) for miniprep and from plates 74 (pSB1A2+rbs+''dxs'', as for M72) and 83 (pSB1A2P+rbs+''appY'', as for M82) for maxipreps. (CF)<br /> |
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:::: '''[[Edinburgh/11_August_2008|Next Entry >]]''' | :::: '''[[Edinburgh/11_August_2008|Next Entry >]]''' |
Latest revision as of 14:40, 28 August 2008
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Week 8
Sunday 10 August 08
- Extracted C. fimi genomic DNA, followed by PCR of rrnB (P61), cenA (P62) and cex (P63) from genomic DNA. (CF)
- Transformed competent cells with L36, pSB1A2+crtY (Plates 104-105) and L37, pSB1A2+rbs+crtY (Plates 106-107). (CF)
- Set-up cultures from plates 102 (pSB1A2+crtB+crtI) and 103 (pSB1A2+glgC-mut1,2) for miniprep and from plates 74 (pSB1A2+rbs+dxs, as for M72) and 83 (pSB1A2P+rbs+appY, as for M82) for maxipreps. (CF)