Team:KULeuven/29 August 2008

From 2008.igem.org

(Difference between revisions)
(Dry Lab)
(Wet Lab)
Line 4: Line 4:
=== Wet Lab ===
=== Wet Lab ===
 +
* A liquid culture was made of the new parts and the ligations we did.
 +
* We also did a colony PCR to test the ligations. They don't seem to be correct.
 +
* Some more digests were made and purified out of gel.
 +
* Ligations were set up.
=== Dry Lab ===
=== Dry Lab ===

Revision as of 19:29, 30 August 2008

  dock/undock dropdown  

Contents

Lab Work

Wet Lab

  • A liquid culture was made of the new parts and the ligations we did.
  • We also did a colony PCR to test the ligations. They don't seem to be correct.
  • Some more digests were made and purified out of gel.
  • Ligations were set up.

Dry Lab

Had another good meeting today. Possibility was raised that adenosine methylation by the dam methylase is inhibiting our XbaI GATC restrictions. Checking it out. JM109 is probably dam+ so XbaI shouldn't cut, while for example JM110 and DM1 (Invitrogen) are dam-. If we want to use XbaI we'll need dam- cells.

Modeling

Nick continued his succesful work on multicellularity in MatLab. He even created a graph that looks like the blueprints of some modern building + created bacteria that could go back in time ;)

We were also able to show the relative effect of the filter by outputting some graphs in MatLab.

Wiki

All parameters on the wiki AND in the MatLab model have been checked and should be correct.

Remarks

<< return to notebook return to homepage >>
< previous friday ← yesterday tomorrow → next monday >